Manitoba Centre for Proteomics and Systems Biology

Are you looking to:

• Analyze and quantify proteins from any sample—be it archaeological or clinical?
  study protein/protein interactions?
• Obtain system-wide insights into phosphorylation-based signaling and kinase activity using only 100,000 cells?
• Use artificial intelligence and machine learning to analyze large datasets?
• Get assistance with uploading your MS project data to data repositories in accordance with publishing guidelines in the field?

The MCPSB can help you with all of the above. Our lab is equipped to perform higher throughput proteomics experiments with hundreds of samples and with minimum amounts of sample material – reach out to discuss specific experiments for example in 96-well format.

Advanced data analysis using artificial intelligence and machine learning

Our advanced data analysis service offers powerful tools for analyzing large omics datasets using machine learning and artificial intelligence. By leveraging these leading-edge technologies, we can unlock valuable insights from complex data sets that would be difficult or impossible to obtain with traditional methods. Our expertise in AI and machine learning allows us to provide reliable and reproducible results, giving researchers the confidence they need to drive their research forward.

Protein-protein interaction

Our protein-protein interaction analysis service provides accurate and comprehensive analysis of protein-protein interactions. We analyze BioID samples and use state-of-the-art technologies to identify and quantify protein-protein interactions with high accuracy and sensitivity. Our expertise in this area gives researchers a deeper understanding into the complex world of protein-protein interactions, paving the way for new advances.

Protein sequence mapping

Our Protein Sequence Mapping Service offers comprehensive and accurate analysis of proteins. Using multiple proteases and state-of-the-art technologies, we perform a comprehensive peptide mapping of proteins to identify and relatively quantify numerous post-translational modifications, truncations, and more. Our expertise enables researchers to deepen their understanding of the complex world of proteins and their interactions.

Post-translational modifications (PTMs)

Our post-translational modifications (PTMS) identification service provides accurate and comprehensive analysis of PTMs across multiple samples. We analyze a wide range of PTMs, such as Ser/Thr/Tyr phosphorylation, proteolytic cleavage sites (N-terminomics), ubiquitination, Cys-nitrosylation, Lys acetylation, N-terminal acetylation, and more. By employing cutting-edge technologies and our expertise, we provide reliable and reproducible results that can lead to groundbreaking discoveries in the field.

Quantitative discovery proteomics

Our quantitative discovery proteomics service identifies and relatively quantifies thousands of proteins across multiple samples using an unbiased approach. By employing state-of-the-art techniques, we help researchers identify significantly regulated proteins and pathways with high accuracy and sensitivity. With our support, researchers can unlock a deeper understanding of the complex world of proteins and their interactions.

Targeted absolute quantitation of proteins and PTMs

Our targeted absolute quantitation of proteins and PTMs service provides reliable and reproducible results for the precise quantitation of predefined targets with high specificity and sensitivity. We develop standardized assays that can be used for biomarker quantitation, allowing researchers to accurately measure the abundance of proteins and PTMs in their samples. This offers researchers greater insights into the role of these targets in disease and other biological processes.

General fee structure

The fees for proteomics services depend largely on the scope and complexity of the project and volume of samples.

For routine analyses: 

Volume discounts may apply for larger projects.

This includes:

• Consultation on experimental design
• Sample preparation including QC by our highly experienced staff
• LC-MS analysis
• Data analysis, including statistical analysis to determine statistically significantly differential proteins or PTM sites, etc. We also provide basic plots for the data
• Follow-up discussion

Supporting ECRs and underrepresented groups
We provide initial analyses especially for new clients and collaborators, e.g., to generate preliminary data for grant applications for a reduced charge or even for free, depending on the project’s complexity and scope. Please reach out to us to discuss.

MS projects are diverse and complex. We offer a free consultation service to discuss your experimental design and sample requirements upfront, so you can get the most out of your MS projects. Our team of experts will work with you to optimize your experimental design, ensuring the best possible outcomes and maximum efficiency. Contact us today to request a consultation and learn more about how we can help you achieve your research goals.

Request a consultation

The Molecular and Cellular Proteomics and Systems Biology laboratory is located in a 10,000 square foot space within the Health Sciences Centre in Winnipeg, Manitoba. We are equipped with state-of-the-art liquid chromatography-mass spectrometry (LC-MS) instrumentation and bioinformatics infrastructure.

Workshops

The Manitoba Centre for Proteomics and Systems Biology (MCPSB) provides a unique opportunity for researchers to gain practical expertise in protein analysis through mass spectrometry and computational proteomics. 

Our hands-on workshops offer a comprehensive learning experience, enabling researchers to explore the latest techniques and approaches in the field. Join us to enhance your research skills and expand your knowledge in proteomics and systems biology.

The cost of the 3-day hands-on workshop is $99 for UM members.

Request training