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IMAGE ANALYSIS Analysis station

  • The analysis station provides researchers with a comprehensive platform to elevate their work, from data processing to visualization. Its high-performance hardware, including the Dell Precision 7920 and Cintiq Pro 22, ensures precision and reliability. Advanced software tools like ZEN Desk 3.1 and Arivis 3D/4D simplify complex image analysis, data extraction, and 3D/4D visualization. Whether refining results in Adobe Photoshop or managing workflows with ZEN Connect, this station empowers researchers to achieve deeper insights efficiently.

    Location: 

    Room 121, Imaging Facility
    University of Manitoba, Bannatyne Campus

  • Analysis Station

Additional information

The analysis station combines high-performance hardware and advanced software to support detailed image analysis and data extraction. 

Below are the key features available for use:

Hardware

  • Computer: Dell Precision 7920, designed for high-performance data processing.
  • Operating system: Windows 10 Enterprise, 64-bit, providing a stable environment for your research tools.
  • Monitor: Samsung 22” monitor for clear, detailed viewing of your analysis.
  • Annotation tablet: Cintiq Pro 22 with stylus for precise annotation and image manipulation.

Software

The software available enhances your ability to process images, extract data, and visualize complex research results. Key tools include:

  • Microsoft Office: For documentation and data organization.
  • ZEN Desk 3.1: A suite for advanced image analysis, offering features such as:
    • Co-localization: Analyzes the overlap of fluorescent markers in your images to investigate molecular interactions.
    • Deconvolution: Enhances image clarity by reducing optical distortions, improving analysis accuracy.
    • Extended Depth of Focus: Improves image clarity across multiple focal planes for detailed results.
    • Intellesis: AI-driven tool that segments images for more efficient data extraction and analysis.
    • ZEN Blue Image analysis: Provides digital analysis to extract quantifiable data from your images for further research insights.
  • ZEN Connect: Helps integrate and manage your data seamlessly across different platforms.
  • Arivis 3D/4D version 4.1: Provides tools for 3D/4D data visualization, useful for analyzing complex datasets.
  • Adobe Photoshop: Advanced image editing software for refining and preparing images for presentations or publications.

ELECTRON MICROSCOPE JEOL JEM-1400 Flash

  • The JEOL JEM-1400 Flash transmission electron microscope offers researchers unparalleled capabilities for high-resolution specimen imaging and 3D tomography. With magnifications up to 1,500,000X, versatile stage control, and accelerating voltages from 60 to 120 kV, it provides exceptional flexibility for detailed exploration. Its 4K CMOS CCD camera ensures precise data capture, while the LaB6 light source delivers superior illumination. Backed by AMT V701 software, it’s an indispensable tool for advancing your research insights.

    Location:

    Room 8, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • Image of an electron microscope

Additional information

The AMT V701 offers advanced electron microscopy and tomography for detailed 3D imaging. 

Below are the key features available for use:

Software

  • AMT V701

Capabilities

  • Electron microscopy for detailed imaging of specimens
  • Tomography for 3D imaging of internal structures

Stage control

  • Manual/Automated - X, Y, Z

Accelerating voltage options

  • 60 kV, 80 kV, 100 kV, 120 kV

Magnifications

  • 50X to 1,500,000X

Camera

  • NANOSPRT43, CMOS CCD, black and white, 4K x 4K pixels

Light source

  • LaB6

Ultramicrotome Leica ARTOS 3D

  • The Leica ARTOS 3D ultramicrotome is an essential tool for researchers requiring precise preparation of biological samples. It offers adjustable sectioning speeds, customizable cutting angles, and back illumination for enhanced visibility. With a section thickness range of 50–500 nanometers and serial sectioning capabilities, it supports high-resolution 3D imaging and structural analysis. Features like knife and specimen rotation and a vibration-isolating table ensure stability and precision, making it ideal for demanding biological research applications.

    Location: 

    Room 109, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • A large piece of lab equipment with an eyepiece and white body.

Additional information

The Leica ARTOS 3D offers advanced sectioning capabilities with adjustable speeds and customizable angles for biological sample preparation. 

Below are the key features available for use:

Adjustable sectioning and return speeds

  • The Leica ARTOS 3D allows precise control over sectioning and return speeds, ensuring adaptability to various biological sample preparation requirements.

Customizable cutting angles

  • Adjustable cutting angles allow the ultramicrotome to be tailored to specific biological research needs.

Back illumination

  • Back illumination enhances visibility of biological samples during sectioning, facilitating real-time monitoring for high-quality results.

Knife and specimen rotation

  • Knife and specimen rotation capabilities offer increased precision and flexibility for optimal positioning of biological samples during preparation.

Performance and capabilities

Section thickness range

  • The Leica ARTOS 3D supports a section thickness range from 50 to 500 nanometers, enabling detailed resolution for biological analyses.

Serial sectioning capabilities

  • The serial sectioning function produces consecutive sections with high precision, essential for 3D imaging and structural analysis of biological samples.

Isolation table

  • The isolation table minimizes vibrations and external disturbances, ensuring stable and accurate sectioning to achieve optimal results for biological research.

Block Trimmer Leica EM Trim 2

  • The Leica EM Block Trimmer is a precision tool tailored for researchers needing high-quality sample preparation. With adjustable feed control (0.1–100 micrometers) and milling speeds up to 20,000 rpm, it delivers flexibility and efficiency for diverse materials. Features like 90° corner accuracy, parallel block faces, and a 60° milling angle range ensure precise, reproducible results. Equipped with tungsten carbide and diamond milling tools, it’s ideal for achieving optimal sample geometry for advanced analyses.

    Location:

    Room 109, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • Image of a piece of equipment with eye pieces and a wide white base.

Additional information

The Leica EM Block Trimmer offers precise sample preparation with adjustable feed control, high milling speeds, and superior tool options. 

Below are the key features available for use:

Adjustable feed control

  • The Leica EM Block Trimmer offers step sizes from 0.1 to 100 micrometers, allowing for fine adjustments to meet your specific sample preparation needs.

High milling speeds

  • Milling speeds range from 300 to 20,000 rpm, providing fast and efficient processing for a broad range of sample types.

90° corner accuracy

  • The block trimmer maintains 90° corner accuracy, ensuring precise sample preparation and optimal geometry for further analysis.

Parallel block face

  • It keeps a perfectly parallel block face, which is critical for consistent and reproducible results when sectioning samples.

Performance and capabilities

Milling angle range

  • With a 60° milling angle range, the Leica EM Block Trimmer offers flexibility to handle a variety of sample shapes and sizes.

Tungsten carbide and diamond milling tools

  • Equipped with tungsten carbide and diamond milling tools, the block trimmer ensures superior control and versatility for preparing high-quality samples across a range of materials.

Ultramicrotome Leica UC7

  • The Leica UC7 ultramicrotome is a powerful tool for researchers requiring precise sample sectioning. With adjustable speeds, fine-tuned cutting angles, and an anti-vibration table, it delivers stable, high-quality results for even the most delicate specimens. A section thickness range of 50–500 nm supports detailed analysis, while features like back illumination and knife/specimen rotation enhance visibility and precision. Tailored for diverse applications, the Leica UC7 ensures flexibility and accuracy for advanced research needs.

    Location:

    Room 8, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • Image of laboratory equipment.

Additional information

The Leica UC7 ultramicrotome offers precise, customizable sectioning with adjustable speeds, cutting angles, and an anti-vibration table. 

Below are the key features available for use:

Adjustable sectioning and return speeds

  • The Leica UC7 allows you to adjust both sectioning and return speeds, offering flexibility for various research needs and ensuring optimal results for different types of samples.

Fine-tuned cutting angles

  • The cutting angle can be precisely adjusted, allowing you to tailor the sectioning process to meet the specific requirements of your specimens.

Back illumination

  • The back illumination system enhances sample visibility, providing clear views of your specimen, even for very thin samples.

Knife and specimen rotation

  • This ultramicrotome includes knife and specimen rotation features, allowing you to adjust the position of the sample for accurate, precise cuts.

Section thickness range

  • With a section thickness range from 50 to 500 nm, the Leica UC7 enables detailed microtomy, providing the precision needed for in-depth analysis.

Stability and performance

Integrated anti-vibration table

  • The integrated anti-vibration table minimizes external disturbances, ensuring stable, reliable sectioning and reproducible results.

ELECTRON MICROSCOPE Phillips CM10

  • The Philips CM10 transmission electron microscope offers high-resolution imaging for detailed specimen analysis. With accelerating voltages from 40–100 kV and magnifications up to 92,000X, it’s ideal for diverse research applications. Features include manual X, Y, Z stage control for precision, a tungsten light source for consistent imaging, and an Advantage HR-B digital CCD camera for high-quality black-and-white images. Supported by AMT 600 software, it streamlines imaging and data capture for your research needs.

    Location:

    Room 14, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • Complex computer equipment.

Additional details

The AMT 600 offers electron microscopy for detailed imaging with adjustable magnifications and accelerating voltage options. 

Below are the key features available for use:

Software

  • AMT 600

Capabilities

  • Electron microscopy for detailed imaging of specimens

Stage control

  • Manual - X, Y, Z

Accelerating voltage options

  • 40 kV, 60 kV, 80 kV, 100 kV

Magnifications

  • LM: 18-530X
  • High magnification: 800-92,000X

Camera

  • Advantage HR-B Digital CCD, black and white

Light source

  • Tungsten

Ultramicrotome Reichert OmU3

  • The Reichert OmU3 ultramicrotome offers precision and adaptability for high-quality sectioning of biological tissues. With adjustable speeds, customizable cutting angles, and a section thickness range of 50–500 nanometers, it ensures detailed and accurate sample preparation. Features like back illumination enhance visibility, while knife and specimen rotation provide flexibility for precise cuts. Ideal for both routine and complex applications, this tool is essential for researchers requiring meticulous sectioning for high-resolution imaging and analysis.

    Location:

    Room 9, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • A large piece of complex lab equipment.

Additional information

The Reichert OmU3 offers adjustable speeds, customizable cutting angles, and enhanced visibility for precise, high-quality sectioning. 

Below are the key features available for use:

Adjustable sectioning and return speeds

  • The Reichert OmU3 allows for precise adjustment of both sectioning and return speeds. This flexibility makes it adaptable to different specimen types and research needs, giving you full control over the cutting process.

Customizable cutting angles

  • With the customizable cutting angles, you can tailor the cutting process to match the specific requirements of your sample preparation, ensuring optimal results for each specimen.

Back illumination for enhanced visibility

  • The back illumination feature improves sample visibility, making it easier to see and section challenging or delicate specimens. This helps you achieve more accurate sectioning for high-quality results.

Knife and specimen rotation

  • The knife and specimen rotation capabilities allow for precise adjustments to the cutting orientation, giving you the flexibility needed for accurate sectioning. This enhances the precision and quality of your work.

Section thickness range

  • The Reichert OmU3 offers a section thickness range from 50 to 500 nanometers, allowing you to create ultra-thin sections ideal for high-resolution imaging and detailed analysis.

IMAGE ANALYSIS Teloview computer

  • This station is optimized for advanced image and data analysis, particularly for telomere FISH analysis using MatLab and TeloView. It offers powerful hardware, including a CoolerMaster computer and a Samsung 22" monitor for clear viewing. With specialized software like MatLab/TeloView, you can efficiently process complex data and images, making it an essential tool for researchers focusing on telomere analysis and other advanced processing tasks. It provides the precision and support needed for detailed investigations.

    Location:

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Teloview Computer

Additional information

The station supports high-performance processing with specialized software for telomere analysis and advanced image processing. 

Below are the key features available for use:

Hardware

  • Computer: CoolerMaster, optimized for high-performance processing tasks.
  • Operating system: Windows 10 Ultimate, 64-bit, ensuring compatibility with various software and tools.
  • Monitor: Samsung 22" monitor for clear and precise viewing of your analysis.

Software

The station is equipped with the following software tools to support your research:

  • MatLab/TeloView: A specialized software combination for telomere FISH analysis and other advanced image processing tasks.
  • Microsoft Office: For documentation, data organization, and report creation.

Brightfield microscopy/image analysis Zeiss Imager A2

  • The Zeiss Imager A2 brightfield microscope is ideal for detailed imaging and instructional use with its dual co-observer ports. It offers high-quality imaging with a variety of Zeiss objectives and advanced ZEN Blue software for image analysis, including tools for colocalization, extended depth of focus, and time-lapse recording. With its dual-camera setup and manual control for precise positioning, it's a versatile tool for both research and educational settings, enabling efficient, high-resolution analysis.

    Location:

    Room 117, Histology Lab
    University of Manitoba (Bannatyne campus)

  • Complex microscope set up with computer monitors.

Additional details

The Zeiss Imager A2 offers advanced imaging capabilities with dual observation arms, precise objective control, and powerful analysis tools. 

Below are the key features available for use:

Hardware

  • Computer: HP Z2 (2019), designed to support complex imaging software.
  • Dual observation arms: Allow multiple users to observe the sample simultaneously.

Software

The following software is available to support your image analysis and processing:

  • ZEN 3 Pro: Offers a range of advanced tools for analyzing and enhancing your images, including:
    • Colocalization: Analyzes the overlap of different fluorescent markers to study molecular interactions.
    • Extended depth of focus: Combines multiple focal planes into a single image with extended depth for clearer results.
    • Time lapse: Records images over time to observe dynamic changes in your sample.
    • ZEN Blue Image analysis: Provides digital analysis to extract quantifiable data from your images.

Objective control

The microscope features manual control for the objective and stage for precise positioning:

  • Manual objective control

Stage control

  • Manual stage control: X, Y, Z axes.

Objectives

The Zeiss Imager A2 is equipped with the following high-quality objectives:

  • Zeiss 1.25X/0,03 ∞/0,17 – EC Plan-Neofluar
  • Zeiss 5X/0,16 Ph 1 ∞/0,17 – EC Plan-Neofluar
  • Zeiss 10X/0,3 ∞/- – EC Plan-Neofluar
  • Zeiss 20X/0,3 ∞/0,17 – EC Plan-Neofluar
  • Zeiss 40X/0,6 Korr ∞/0-1.5 – LD Plan-Neofluar
  • Zeiss 100X/1,3 oil Ph 3 ∞/0,17 – EC Plan-Neofluar

Camera

The system is equipped with two cameras for capturing detailed images:

  • AxioCam IcC 5: Colour camera for high-quality imaging.
  • AxioCam 208: Colour camera for enhanced image clarity.

SIM Zeiss ELRYA PS.1

  • The Zeiss ELYRA PS.1 fluorescence super-resolution microscope offers high-resolution imaging with advanced structured illumination and photoactivated localization microscopy (PALM). Equipped with automated objectives, stage control, and powerful ZEN software, it enables precise 3D rendering, co-localization, and time-lapse imaging. This system is ideal for detailed analysis of fluorescently labeled samples, providing unparalleled clarity and super-resolution imaging for advanced research in cellular and molecular biology.

    Location:

    Room 125A, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • Complex microscope set up with computer monitors.

Additional details

The system offers advanced imaging with automated stage and objective control, 3D rendering, super-resolution imaging, and time-lapse capture. 

Below are the key features available for use:

Hardware

  • Computer: HP Z6 P4 (2022)

Software

  • ZEN D Black
    • 3D rendering: Creates three-dimensional images from multiple focal planes
    • Co-localization: Analyzes the overlap of different fluorescent markers
    • Extended depth of focus: Enhances image clarity across different focal planes
    • Fluorescence: Enables observation of fluorescently labelled samples
    • PALM: Photoactivated localization microscopy for super-resolution imaging
    • SIM: Structured illumination microscopy for enhanced resolution
    • Time lapse: Captures changes in samples over time
    • Software autofocus: Maintains sample focus over time
    • Z-stack: Constructs a 3D image by stacking images taken at different focal depths
  • ZEN 2.3 Blue
    • ZEN Blue image analysis: Digital analysis to extract quantifiable data from your images

Objective control

  • Automated

Stage control

  • Automated – X, Y, Z

Objectives

  • Zeiss N-Achroplan 10x/1.025 M27
  • Zeiss Plan-Apochromat 20x/1.08 M27
  • Zeiss Plan-Apochromat 40x/1.4 Oil DIC M27
  • Zeiss Plan-Apochromat 63x/1.40 Oil DIC M27
  • Zeiss Alpha Plan-Apochromat 100x/1.46 Oil DIC M27 Elyra
  • Zeiss Alpha Plan-Apochromat 100x/1.57 Oil DIC Korr M27

Camera

  • Black and white

Light source lasers

  • X-cite
  • HR Diode 405-50nm
  • HR Diode 488-100nm
  • HR DPSS 561-100nm
  • HR Diode 642-100nm

Filters

  • Zeiss filter set 77HE
  • Analyzer module: Pol ACR

Filter Wheel 1:

  • BP 420-480 + BP 570-640 + LP 740
  • BP 420-480 + BP 495-550 + LP 650
  • LBF 561-642
  • LBF 488/561

Filter Wheel 2:

  • BP 420-480 + LP 750
  • BP 495-550 + LP 750
  • BP 570-620 + LP 750
  • LP 655     

Grids

  • 23m
  • 28m
  • 34m
  • 42m
  • 51m

BRIGHTFIELD/FLUORESCENCE STEREO Zeiss Lumar V.12

  • The Zeiss Lumar V.12 is a versatile stereo microscope ideal for imaging larger specimens in both brightfield and fluorescence modes. With manual and automated controls, it offers high-quality imaging using a variety of objectives and advanced ZEN software. Features like extended depth of focus, time-lapse, and Z-stack allow for in-depth analysis of specimens with enhanced clarity and 3D rendering. This system is perfect for researchers needing detailed imaging of large, fluorescently labeled samples.

    Location:

    Room 117, Histology Lab
    University of Manitoba (Bannatyne campus)

  • Complex microscope set up with computer monitors.

Additional details

The Zeiss Lumar V.12 is a brightfield and fluorescence stereo microscope designed for imaging larger specimens.

Below are the key features available for use:

Hardware

  • Computer: CompuData

Software

  • ZEN 2
    • Brightfield: For viewing stained or naturally coloured specimens
    • Extended depth of focus: Enhances image clarity across different focal planes
    • Fluorescence: Allows for the observation of fluorescently labelled samples
    • Time lapse: Captures changes in samples over time
    • Z-stack: Constructs three-dimensional images from multiple focal planes

Objective control

  • Manual exchange

Stage control

  • Manual rotating – X, Y
  • Automated Z

Objectives

  • Zeiss 0.8x 80mm
  • Zeiss 1.2x 47mm
  • Zeiss 1.5x 30mm

Camera

  • AxioCam HRm – Black and white
  • AxioCam ICc 3 – Colour

Light source

  • HBO 100 mercury bulb

Filters

  • Zeiss filter set 02
  • Zeiss filter set 38
  • Zeiss filter set 43
  • Zeiss filter set 49

Brightfield/fluorescence Zeiss Imager M2

  • The Zeiss Imager M2 is a versatile microscope ideal for imaging histological stains and fluorescent samples. With high-quality brightfield and fluorescence imaging, it supports dynamic analysis through features like extended depth of focus, time-series imaging, and co-localization. The system includes advanced software tools for detailed analysis, multiple objectives for varying magnification, and two camera options for colour and high-resolution imaging, making it perfect for a range of research applications in biology and material science.

    Location:

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Zeiss M2

Additional details

The Zeiss Imager M2 is a brightfield/fluorescent microscope designed for high-quality imaging of histological stains. 

Below is a breakdown of its key features:

Hardware

  • Computer: HP Z6: A high-performance computer equipped to manage advanced imaging software and handle large datasets efficiently.

Software

  • ZEN 3 Pro:
    • A versatile software suite that includes various tools for imaging and analysis, tailored for detailed research:
    • Brightfield: For viewing stained or naturally coloured specimens.
    • Co-localization: Analyzes the overlap of different fluorescent markers to study complex interactions.
    • Differential interference contrast (DIC): Provides enhanced contrast for transparent specimens, making subtle structures more visible.
    • Extended depth of focus: Combines images from multiple focal planes into one, improving clarity across depth.
    • Fluorescence: Enables imaging of fluorescently labelled samples, useful for specific marker detection.
    • Time series: Captures a series of images over time to observe dynamic biological processes.
    • ZEN Blue image analysis: Provides digital tools for extracting quantifiable data from images for in-depth analysis.

Objective control

  • Manual objective control: Provides fine-tuning of objectives for precise imaging.

Stage control

  • Manual stage control (X, Y, Z axes): Allows for optimal positioning and movement of the specimen for accurate imaging.

Objectives

The microscope is equipped with a selection of Zeiss objectives for varying magnification needs:

  • Zeiss 2.5x/0,075 ∞/0,17 – EC Plan-Neofluar
  • Zeiss 5x/0,16 ∞/0,17 – EC Plan-Neofluar
  • Zeiss 10x/0,3 ∞/- – EC Plan-Neofluar
  • Zeiss 20x/0,5 ∞/0,17 – EC Plan-Neofluar
  • Zeiss 40x/0,75 ∞/0,17 – EC Plan-Neofluar
  • Zeiss 100x/1,3 oil ∞/0,17 – EC Plan-Neofluar

Cameras

  • AxioCam HRc: Colour camera designed for high-resolution imaging of specimens.
  • AxioCam MRm: Black-and-white camera suited for high-resolution imaging and capturing detailed structural information.

Light source

The Colibri light source provides multiple LED wavelengths to enhance imaging quality:

  • 365nm LED
  • 470nm LED
  • 555nm LED
  • 590nm LED

Filters

  • Zeiss filter set 62HE: A filter set designed for fluorescence applications, providing specific excitation and emission wavelengths for optimal imaging.

BRIGHTFIELD/FLUORESCENCE Zeiss Imager M2 Scan

  • The Zeiss Imager M2 Scan is a high-performance microscope designed for large-scale imaging and advanced sample analysis. With multi-slide scanning, it offers both brightfield and fluorescence imaging, enabling detailed studies of complex specimens. Key features include 3D rendering, co-localization, deconvolution, and AI-powered Intellesis segmentation. Its automated and manual controls, multiple objectives, and high-quality cameras make it ideal for dynamic, large-volume research.

    Location:

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Electron microscope

Additional details

The Zeiss Imager M2 Scan is a brightfield and fluorescence microscope with multi-slide scanning capabilities, 

Below are the key features available for use:

Hardware

  • Computer: HP Z6
  • Apotome
  • 4-slide holder

Software

  • ZEN 3.8 Pro
    • 3D rendering: Creates 3D images from multiple focal planes.
    • Brightfield: For viewing stained or naturally colored specimens.
    • Co-localization: Analyzes the overlap of different fluorescent markers.
    • Deconvolution: Enhances clarity by reducing optical distortions.
    • Extended depth of focus: Enhances image clarity across different focal planes.
    • Fluorescence: Enables observation of fluorescently labeled samples.
    • Intellesis: AI segmentation tool for image analysis.
    • Software autofocus: Maintains image focus over time.
    • Tiling: Creates large composite images from multiple fields of view.
    • Time series: Captures a series of images over time for dynamic studies.
    • Z-stack: Constructs three-dimensional images from multiple focal planes.

Objective control

  • Manual/automated

Stage control

  • Manual/automated - X, Y, Z

Objectives

  • Zeiss 2.5x/0.085 ∞/0.17 – EC Plan-Neofluar
  • Zeiss 5x/0.16 ∞/0.17 – EC Plan-Neofluar
  • Zeiss 10x/0.3 ∞/- – EC Plan-Apochromat
  • Zeiss 20x/0.8 ∞/0.17/OFN25 – EC Plan-Apochromat
  • Zeiss 63x/1.4 oil ∞/0.17 – Plan-Apochromat

Camera

  • AxioCam 305 - colour
  • AxioCam 705 - black and white

Light source (Colibri)

  • 385nm LED
  • 430nm LED
  • 475nm LED
  • 555nm LED
  • 590nm LED
  • 630nm LED
  • 735nm LED

Filters

  • Zeiss Filter Set 38HE
  • Zeiss Filter Set 49HE
  • Zeiss Filter Set 63HE
  • Zeiss Filter Set 112HE

Cell culture/live cell Zeiss Observer D1

  • The Zeiss Imager D1 is ideal for live cell imaging, offering brightfield, fluorescence, and phase contrast capabilities. With its manual objective control and versatile stage inserts, it's perfect for a range of biological samples. Key features include time-lapse imaging, Z-stack for 3D reconstruction, and advanced fluorescence filters. Its high-quality black-and-white camera and multiple light sources ensure precise and dynamic imaging, making it an excellent choice for time-sensitive biological research.

    Location:

    Room 103C, Cell Culture
    University of Manitoba (Bannatyne campus)

  • Complex microscope set up with computer monitors.

Additional information

The Zeiss Imager D1 is a brightfield/fluorescent microscope designed for live cell imaging. 

Below are the key features available for use:

Hardware

  • Computer: Dell Precision
  • Stage inserts: Multiwell plate, T25 flask, T75 flask, Petri dish, microscope slide

Software

  • ZEN 3 Pro
    • Brightfield: For viewing stained or naturally coloured specimens
    • Phase contrast: Enhances unstained samples for imaging
    • Fluorescence: For observing fluorescently labelled samples
    • Tiling: Captures overlapping images to cover a larger area (manual stage)
    • Time lapse: Records images over time to observe changes
    • Z-stack: Constructs a 3D image by stacking images at different depths (manual stage)

Objective control

  • Manual

Stage control

  • Manual – X, Y, Z

Objectives

  • Zeiss 5x/0.16 Ph1 ∞/0.17 – EC Plan-Neofluar
  • Zeiss 10x/0.3 Ph1 ∞/- – EC Plan-Neofluar
  • Zeiss 20x/0.4 Ph1 Ph2-Korr ∞/0-1.5 – LD Plan-Neofluar
  • Zeiss 40x/0.6 Ph1 Ph2-Korr ∞/0-1.5 – LD Plan-Neofluar

Camera

  • AcioCam MRm – Black and white

Light sources

  • Colibri:
    • 365nm LED
    • 470nm LED
    • 555nm LED
    • 590nm LED
  • Xylis:
    • 350-750nm

Filters

  • Zeiss filter set 02
  • Zeiss filter set 10
  • Zeiss filter set 20
  • Zeiss filter set 43HE
  • Zeiss filter set 46HE
  • Zeiss filter set 62HE

Cell culture/live cell Zeiss Observer 7

  • The Zeiss Observer 7 is ideal for extended live cell imaging, offering advanced phase contrast and fluorescence capabilities. It features automated objective and stage control, environmental controls (CO2, humidity, temperature), and high-resolution imaging tools like Z-stack, 3D rendering, and automated software autofocus. This microscope is perfect for capturing dynamic cellular processes over extended time periods with precise control and enhanced image clarity.

    Location:

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Complex microscope set up with computer monitors.

Additional information

The Zeiss Observer 7 is a phase contrast/fluorescent microscope designed for live cell imaging, particularly suitable for extended time point imaging.

Below are the key features available for use:

Hardware

  • Computer: HP Z6 (2021)
  • Stage inserts: Multiwell plate, T25 flask, T75 flask, Petri dish, microscope slide

Software

  • ZEN 3.7
    • 3D rendering: Creates 3D images from multiple focal planes
    • Co-localization: Analyzes the overlap of different fluorescent markers
    • Extended depth of focus: Enhances image clarity across different focal planes
    • Fluorescence: Observes fluorescently labelled samples
    • Phase contrast: Enhances unstained samples for imaging
    • Software autofocus/definite focus: Maintains image focus over time
    • Tiling: Captures overlapping images to cover a larger area
    • Time lapse: Records images over time to observe changes
    • ZEN Blue image analysis: Digital analysis to extract quantifiable data from your images
    • Z-stack: Constructs a 3D image by stacking images at different depths

Objective control

  • Automated

Stage control

  • Automated – X, Y, Z

Environmental controls

  • CO2: Controls for carbon dioxide levels
  • Humidity: Monitors and adjusts humidity levels
  • Temperature: Regulates temperature for stable conditions

Objectives

  • Zeiss 5x/0.16 Ph1 ∞/0.17 – EC Plan-Neofluar
  • Zeiss 10x/0.3 Ph1 ∞/- – EC Plan-Neofluar
  • Zeiss 20x/0.4 Ph1 Ph2-Korr ∞/0-1.5 – LD Plan-Neofluar
  • Zeiss 40x/1.3 Oil DIC (UV) VIS-IR M27 – Plan-Apochromat
  • Zeiss 40x/1.2 Imm Korr DIC M27 – LD LCI Plan-Apochromat

Camera

  • AxioCam MRm – Black and white

Light sources

  • Colibri:
    • 365nm LED
    • 470nm LED
    • 555nm LED
    • 590nm LED

Filters

  • Zeiss filter set 20
  • Zeiss filter set 38
  • Zeiss filter set 49
  • Zeiss filter set 50HE
  • Zeiss filter set 62HE

Brightfield/Fluorescence Zeiss Imager Z1

  • The Zeiss Imager Z1 offers advanced brightfield and fluorescence imaging with 3D capabilities, ideal for complex research. It includes features like deconvolution for enhanced clarity, AI-powered image analysis, and software autofocus for extended imaging sessions. With versatile objective and stage controls, it provides precise imaging of various specimen types, including transparent and fluorescent samples. This system is perfect for researchers requiring high-resolution, multi-dimensional imaging and quantitative analysis across multiple focal planes.

    Location:

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Zeiss Z1

Additional information

The Zeiss Imager Z1 is a brightfield and fluorescence microscope with 3D imaging capabilities, designed for advanced research applications.

Below are the key features available for use:

Hardware

  • Computer: HP Z6 (2019)

Software: ZEN 3 Pro features

  • 3D rendering: creates 3D images from multiple focal planes
  • Brightfield: suitable for viewing stained or naturally coloured specimens
  • Co-localisation: analyses overlap between different fluorescent markers
  • Deconvolution: enhances image clarity by reducing optical distortions
  • DIC (differential interference contrast): ideal for transparent specimens
  • Extended depth of focus: improves clarity across various focal planes
  • Fluorescence: observes fluorescently labelled samples
  • Intellesis: AI-powered segmentation tool for image analysis
  • Positions: enables setting and recalling specific sample positions
  • Software autofocus: maintains image focus over extended periods
  • Tiling: captures overlapping images to create a larger composite image
  • Time lapse: records image sequences over time to monitor changes
  • ZEN Blue image analysis: provides digital tools to extract quantifiable data
  • Z-stack: constructs 3D images by stacking multiple focal plane images

Objective control

  • Manual or automated control available

Stage control

  • Manual or automated x, y, z axis movement

Objectives

  • Zeiss 10x/0.3 ∞/- – Plan-Neofluar
  • Zeiss 20x/0.8 ∞/0.17 – Plan-Neofluar
  • Zeiss 40x/0.75 ∞/0.17 – EC Plan-Neofluar
  • Zeiss 40x/1.3 oil DIC ∞/0.17 – EC Plan-Neofluar
  • Zeiss 63x/1.4 oil DIC ∞/0.17 – Plan-Neofluar
  • Zeiss 100x/1.3 oil M27 – EC Plan-Neofluar

Camera

  • AxioCam 105 (colour)
  • AxioCam MRm (black and white)

Light source (Colibri LEDs)

  • 365 nm
  • 470 nm
  • 555 nm
  • 590 nm

Filters

  • Zeiss Filter Set 20HE
  • Zeiss Filter Set 38HE
  • Zeiss Filter Set 43HE
  • Zeiss Filter Set 49HE
  • Zeiss Filter Set 50HE
  • Zeiss Filter Set 62HE

BRIGHTFIELD/FLUORESCENCE Zeiss Imager Z2

  • The Zeiss Imager Z2 is an advanced microscope designed for brightfield and fluorescence imaging, with capabilities for 3D imaging and precise optical sectioning. It features the ApoTome.2 for enhanced clarity, a four-slide holder for multi-sample imaging, and the ZEN 3 Pro software suite for 3D rendering, time-lapse imaging, and quantitative analysis. With both manual and automated controls, it provides flexibility for high-resolution imaging of complex biological specimens across multiple focal planes.

    Location:

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Zeiss Z2

Additional information

The Zeiss Imager Z2 is a brightfield and fluorescence microscope with advanced 3D imaging capabilities. 

Below are the key features available for use:

Hardware

  • Computer: HP Z240 (2019)
  • ApoTome.2: Enhances optical sectioning
  • 4-slide holder: Allows simultaneous imaging of up to four slides

Software: ZEN 3 Pro

The ZEN 3 Pro software suite offers comprehensive imaging features, including:

  • 3D rendering: creates three-dimensional images from multiple focal planes
  • Brightfield: ideal for stained or naturally coloured specimens
  • DIC (differential interference contrast): enhances the visibility of transparent samples
  • Extended depth of focus: improves clarity by combining images from different focal planes
  • Fluorescence: captures images of fluorescently labelled samples
  • Positions: allows users to set and recall specific sample locations
  • Software autofocus: maintains sharp focus over time
  • Tiling: merges overlapping images for large-area imaging
  • Time lapse: records changes in samples over time
  • Z-stack: constructs 3D images by stacking images from varying depths

Objective control

  • Manual/automated: facilitates precise adjustments

Stage control

  • Manual/automated (X, Y, Z axes): enables exact positioning and movement

Objectives

  • Zeiss 2.5x/0.075 ∞/0.17 – EC Plan-Neofluar
  • Zeiss 10x/0.3 ∞/- – Plan-Neofluar
  • Zeiss 20x/0.8 ∞/0.17 – Plan-Neofluar
  • Zeiss 40x/0.75 ∞/0.17 – EC Plan-Neofluar
  • Zeiss 63x/1.4 oil DIC ∞/0.17 – Plan-Neofluar

Camera options

  • AxioCam 208 (colour)
  • AxioCam MRm (black and white)

Light source: Colibri LED

  • 385 nm
  • 430 nm
  • 475 nm
  • 555 nm
  • 590 nm
  • 630 nm
  • 735 nm

Filters

  • Zeiss filter set 2HE
  • Zeiss filter set 20HE
  • Zeiss filter set 38HE
  • Zeiss filter set 43HE
  • Zeiss filter set 49HE
  • Zeiss filter set 50HE
  • Zeiss filter set 62HE
  • Zeiss filter set 112HE

Contact us

Histology Services, Imaging Facility, Electron Microscopy Platform
Room 119, 745 Bannatyne Avenue
Basic Medical Science Building
University of Manitoba (Bannatyne campus)
Winnipeg, MB R3E 0J9

histology_services@umanitoba.ca
204-789-3508