Costs

The imaging facility is run on a cost-recovery basis and the following fees apply:

UM community: $50/hour if self-imaged and $75/hour if staff does the imaging. 

External users: $75/hour if self-imaged and $100/hour if staff does the imaging. 

Training: $75/hour for UM community and $100/hour for external users.

Please note that the ELYRA PS.1 must be operated by facility staff. It is billed at  $75/hour for members of the UM community and $120.00 per hour for external users.

Training and access

  • Training

    Training is mandatory for all users

    It is up to histology staff, representative trainers, and the department head/platform director to decide whether the user has demonstrated adequate understanding of the instrument to be granted unsupervised access.

    Upon completion of training, users must pass a practical test to be granted full privileges.

  • Request for training/access

    Please complete the request form.

    Training and access request form (PDF)

Facility use guidelines

New users

All new users are required to contact histology staff to arrange necessary training and initiate fee payments when applicable.

Training

Training is mandatory for all users by histology staff or Zeiss representatives.

It is up to histology staff, representative trainers, and the department head/platform director to decide whether the user has demonstrated adequate understanding of the instrument to be granted unsupervised access.

Upon completion of training users must pass a practical test to be granted full privileges.

Booking time on instruments

Booking will be done using calendars next to each piece of equipment.

It is the responsibility of the user to ensure time booked is honored.

Billing will be calculated based on the booked time and logbook records.

If users do not cancel their unused time they will be billed.

Failure to honour booked times may result in loss of privileges of the instrument.

If users do not arrive within 30 minutes of the booked time the booking becomes void, and the equipment is considered free for use by another user.

Facility use

Users must strictly follow the startup and shutdown procedures for each instrument.

Gloves are not permitted on any of the instruments or computers.

All users are required to fill in the logbook for each instrument during every use. No exceptions.

Instruments must be left in pristine condition when finished.

Everything must be turned off, covered, and oil lenses cleaned, and any rubbish discarded.

Data storage and file transfer

Data should be stored directly to the r: drive for each individual lab when possible.

Data can be temporarily stored on the hard drives of the instruments in the d: drive.

The facility is not responsible for data stored on these hard drives; leaving data here is at the user’s own risk.

Data left here indefinitely will be deleted without warning.

USB flash drives are not permitted unless newly purchased from the histology lab. If you require a new USB, CD/DVD see histology staff.

Users are responsible for their own data.

Problems

Please notify histology staff immediately of any problems.

This includes supply depletion (oil, lens paper, cleaning solution), software issues, burnt out bulbs or filaments or any other issues associate with use of the instruments.

After hours / weekends

If problems occur after hours, on weekends, or on holidays please shut down the equipment, place a sign so no one else uses it, and contact histology staff at histology_services@umanitoba.ca. For emergencies call 555 for security or 911.

IMAGE ANALYSIS Analysis station

  • The analysis station offers analysis, colocalization, deconvolution, extended depth of focus, intellesis and measurement.

    Hardware

    Windows 10 Enterprise, 64 Bit with Samsung, 22” monitor

    Software

    • Microsoft Office
    • ZEN Desk 3.1

    Location

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Analysis Station

Phillips CM10

  • The Phillips CM10 is an electron microscope.

    Software

    AMT 600

    Objective control

    Manual

    Stage control

    Manual

    Location 

    Room 14, Anatomy Department
    University of Manitoba (Bannatyne campus)

  • Complex computer equipment.

Additional details

Capabilities

Electron microscopy

Magnification
  • 800
  • 1100
  • 1450
  • 1950
  • 2600
  • 3400
  • 4600
  • 5800
  • 7900
  • 10500
  • 13500
  • 19000
  • 25000
  • 34000
  • 46000
  • 64000
  • 92000
Light source

Tungsten

Camera

Advantage HR-B Digital CCD - Black and White

IMAGE ANALYSIS Teloview computer

  • Hardware

    Windows 10 Ultimate, 64 Bit with Samsung, 22” monitor

    Software

    MatLab/TeloView 

    Location

    Room 121, Imaging Facility
    University of Manitoba (Bannatyne campus)

  • Teloview Computer

Brightfield microscopy/image analysis Zeiss A2

  • The Zeiss A2 is a bright field microscope with dual co-observer ports.

    It offers analysis and bright field.

    Location

    Room 117, Histology Lab
    University of Manitoba (Bannatyne campus)

  • Complex microscope set up with computer monitors.

Additional details

Software

ZEN 3 Pro

Objective control

Manual

Stage control

Manual - X, Y

    Objectives
    • Zeiss 1.25X/0,03 ∞/0,17 – EC Plan-Neofluar
    • Zeiss 5x/0,16 Ph1 ∞/0 17 – EC Plan- Neofluar
    • Zeiss 10x/0, 3 Ph1 ∞/- – EC Plan- Neofluar
    • Zeiss 20X/0, 4 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    • Zeiss 40X/0, 6 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    • Zeiss 100X/1,3 oil Ph 3 ∞/0,17 – EC Plan- Neofluar
    Light source
    • LED
    Camera
    • AxioCam 208 – colour
    • AxioCam ICc 5 – colour

    SIM Zeiss ELRYA PS.1

    • The Zeiss ELRYA PS.1 is a fluorescence supperresolution structured illumination microscope.

      Location
      Room 125A, Anatomy Department
      University of Manitoba (Bannatyne Campus)

    • Complex microscope set up with computer monitors.

    Additional details

    Software

    ZEN D

    Objective control

    Automated

    Stage control

    Automated X, Y, Z

    Capabilities
    • 3D rendering
    • co-localization
    • fluorescence
    • PALM
    • SIM
    • z-stack
    Objectives
    • Zeiss Alpha Plan-Apochromat 100X/1.46 Oil DIC M27 Elyra
    • Zeiss Alpha Plan-Apochromat 100X/1.57 Oil DIC Korr M27
    • Zeiss N-Achroplan 10X/1.025 M27
    • Zeiss Plan_apochromat 20X/1.08 M27
    • Zeiss Plan_apochromat 40X/1.4 Oil DIC M27
    • Zeiss Plan_apochromat 63X/1.40 Oil DIC M27
    Light source
    • Mercury bulb
    Filters

    Zeiss Filter Set 77HE

    Light source

    Laser         

    • HR Diode 405-50 (dapi)         
    • HR Diode 488-100 (green)         
    • HR DPSS 561-100 (red)         
    • HR Diode 642-100 (far red)
    Filters
    • Analyzer module Pol ACR
    • Filter Wheel 1
      • BP 420-480 + BP 570-640 + LP 740
      • BP 420-480 + BP 495-550 + LP 650                   
      • LBF 561-642
      • LBF 488/561
    • Filter Wheel 2
      • BP 420-480 + LP 750
      • BP 495-550 + LP 750                                          
      • BP 570-620 + LP750
      • LP 655
    Grids
    • 23m
    • 28m
    • 34m
    • 42m
    • 51m
    Camera

    Black and white

    Fluorescence stereo Zeiss Lumar V.12

    • The Zeiss Lumar V.12 is a bright field and fluorescence stereo microscope.

      Location

      Room 117, Histology Lab
      University of Manitoba (Bannatyne campus)

    • Complex microscope set up with computer monitors.

    Additional details

    Software

    ZEN 2

    Objective control

    Manual exchange

    Stage control

    Rotating - X, Y

    Capabilities
    • bright field
    • extended depth of focus
    • fluorescence
    • time lapse
    • z-stack
    Objectives
    • Zeiss 0.8X 80mm
    • Zeiss 1.2X 47mm
    • Zeiss 1.5X 30mm
    Light source

    HBO 100 mercury bulb

    Filters
    • Zeiss Filter Set 20
    • Zeiss Filter Set 38HE
    • Zeiss Filter Set 43HE
    • Zeiss Filter Set 49
    Camera

    AxioCam HRm – black and white

    AxioCam ICc 3 – colour

    FLUORESCENCE/Brightfield STEREO Zeiss Lumar V.12

    • The Zeiss Lumar V.12 is a fluorescence stereo microscope

      Location

      Room 121, Imaging Facility
      University of Manitoba (Bannatyne campus)

    • Zeiss Lumar V.12

    Additional information

    Software

    AxioVision

    Objective Control

    Manual exchange

    Stage Control

    Rotating – X, Y

    Capabilities
    • Extended depth of focus
    • Fluorescence
    • Time lapse
    • Z-stack
    Objectives
    • Zeiss 0.8X 80mm
    • Zeiss 1.2X 47mm
    • Zeiss 1.5X 30mm
    Light Source

    HBO 100 mercury bulb

    Filters
    • Zeiss Filter Set 49
    • Zeiss Filter Set 20HE
    • Zeiss Filter Set 38HE
    Camera

    AxioCam HRm – black and white

    Brightfield/fluorescence Zeiss M2

    • The Zeiss M2 is a bright field and fluorescence microscope.

      Location

      Room 121, Imaging Facility
      University of Manitoba (Bannatyne campus)

    • Zeiss M2

    Additional details

    Software

    ZEN 3 Pro

    Objective control

    Manual/automated

    Stage control

    Manual/rotating - X, Y

    Capabilities
    • analysis
    • bright field
    • co-localization
    • fluorescence
    Objectives
    • Zeiss 2.5x/0,075 ∞/0,17 – EC Plan- Neofluar
    • Zeiss 5x/0,16 Ph1 ∞/0 17 – EC Plan- Neofluar
    • Zeiss 10x/0, 3 Ph1 ∞/- – EC Plan- Neofluar
    • Zeiss 20X/0, 4 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    • Zeiss 40X/0, 6 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    • Zeiss 100X/1,3 oil Ph 3 ∞/0,17 – EC Plan- Neofluar
    Light source
    • Colibri
      • 365nm LED
      • 470nm LED
      • 555nm LED
      • 590nm LED
    • LED
    Filters
    • DIC
    • Zeiss Filter Set 38HE
    • Zeiss Filter Set 43HE
    • Zeiss Filter Set 49HE
    • Zeiss Filter Set 50HE
    • Zeiss Filter Set 62HE - colibri only
    Camera
    • AxioCam HRc – colour
    • AxioCam MRm – black and white

    Cell culture/live cell Zeiss Observer D1

    • The Zeiss Observer D1 is a bright field/phase contrast and fluorescence live cell microscope.

      Location

      Room 103C, Cell Culture
      University of Manitoba (Bannatyne campus)

    • Complex microscope set up with computer monitors.

    Additional information

    Software

    ZEN 3 Pro

    Objective control

    Manual

    Stage control

    Automated

    Capabilities
    • bright field
    • fluorescence
    • phase contrast
    • tiling
    • time series
    • z-stack
    Objectives
    • Zeiss 5x/0,16 Ph1 ∞/0 17 –  EC Plan- Neofluar
    • Zeiss 10x/0, 3 Ph1 ∞/- –  EC Plan- Neofluar
    • Zeiss 20X/0, 4 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    • Zeiss 40X/0, 6 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    Light source
    • Colibri    
      • 365nm LED    
      • 470nm LED    
      • 555nm LED    
      • 590nm LED
    • LED
    Filters
    • Zeiss Filter Set 02
    • Zeiss Filter Set 10
    • Zeiss Filter Set 20
    • Zeiss Filter Set 43HE
    • Zeiss Filter Set 62HE - Colibri only
    Camera

    AxioCam MRm - Black and White

    Cell culture/live cell Zeiss Observer 7

    • The Zeiss Observer 7 is a bright field/phase contrast and fluorescence live cell microscope.

      Location

      Room 121, Imaging Facility
      University of Manitoba (Bannatyne campus)

    • Complex microscope set up with computer monitors.

    Additional information

    Software

    ZEN 3 Pro

    Objective control

    Automated

    Stage control

    Automated

    Capabilities
    • bright field
    • fluorescence
    • phase contrast
    • tiling
    • time series
    • z-stack
    Environmental controls
    • CO2
    • humidity
    • temperature
    Objectives
    • Zeiss 5x/0,16 Ph1 ∞/0 17 –  EC Plan- Neofluar
    • Zeiss 10x/0, 3 Ph1 ∞/- –  EC Plan- Neofluar
    • Zeiss 20X/0, 4 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    • Zeiss 40X/0, 6 Ph1 Ph2-Korr ∞/0-1, 5 – LD Plan-Neofluar
    Light source
    • LED
    • Colibri    
      • 365nm LED    
      • 470nm LED    
      • 555nm LED    
      • 590nm LED
    Filters
    • Zeiss Filter Set 02
    • Zeiss Filter Set 10
    • Zeiss Filter Set 20
    • Zeiss Filter Set 43HE
    • Zeiss Filter Set 62HE - Colibri only
    Camera

    AxioCam MRm - Black and White

    Brightfield/Fluorescence Zeiss Z1

    • The Zeiss Z1is a fluorescence microscope with 3D imaging.

      Location

      Room 121, Imaging Facility
      University of Manitoba (Bannatyne campus)

    • Zeiss Z1

    Additional information

    Software

    ZEN 3 Pro

    Objective control

    Manual/automated

    Stage control

    Manual/automated - X, Y, Z

    Capabilities
    • 3D rendering
    • deconvolution
    • DIC
    • fluorescence
    • image analysis
    • microarray
    • mosaix
    • positions
    • tiling
    • time lapse
    • z-stack
    Objectives
    • Zeiss 10x/0,3 ∞/- – Plan- Neofluar
    • Zeiss 20X/0,8 ∞/0,17 – Plan-Neofluar
    • Zeiss 40X/0,75 ∞/0,17 – EC Plan-Neolfuar
    • Zeiss 40X/1,3 oil DIC ∞/0,17 – EC Plan-Neolfuar
    • Zeiss 63X/1,4 oil DIC ∞/0,17 – Plan-Neofluar
    Light source
    • Colibri
      • 365nm LED
      • 470nm LED
      • 555nm LED
      • 590nm LED
    • LED
    Filters
    • DIC
    • Zeiss Filter Set 38HE
    • Zeiss Filter Set 43HE
    • Zeiss Filter Set 49HE
    • Zeiss Filter Set 50HE
    • Zeiss Filter Set 62HE - colibri only
    Camera
    • AxioCam 105 – Colour
    • AxioCam MRm – Black and White

    BRIGHTFIELD/FLUORESCENCE Zeiss Z2

    • The Zeiss Z2 is a fluorescence microscope with 3D imaging and apotome.

      Location

      Room 121, Imaging Facility
      University of Manitoba (Bannatyne campus)

    • Zeiss Z2

    Additional information

    Software

    ZEN 3 Pro

    Objective control

    Manual/automated

    Stage control

    Manual/automated - X, Y, Z

    Capabilities
    • 3D rendering
    • DIC
    • extended depth of focus
    • fluorescence
    • tiling
    • time lapse
    • z-stack
    Objectives
    • Zeiss 2.5X/0,75 ∞/0,17 – EC Plan-Neofluar
    • Zeiss 5x/0,16 ∞/0,17 – EC Plan-Neofluar
    • Zeiss 10x/0,3 ∞/0,17 – EC Plan-Neofluar
    • Zeiss 20X/0,5 ∞/0,17 – EC Plan-Neofluar
    • Zeiss 40X/0,75 ∞/0,17 – EC Plan-Neolfuar
    • Zeiss 63X/1,4 oil DIC ∞/0,17 – Plan-Neofluar
    Light source

    Colibri

    • 365nm LED
    • 470nm LED
    • 555nm LED
    • 590nm LED
    Filters
    • Zeiss Filter Set 20HE
    • Zeiss Filter Set 38HE
    • Zeiss Filter Set 43
    • Zeiss Filter Set 49
    • Zeiss Filter Set 50
    • Zeiss Filter Set 62HE – colibri onlyDIC
    Camera
    • AxioCam 208 – Colour
    • AxioCam MRm – Black and White

    Contact us

    Histology Services, Imaging Facility, Electron Microscopy Platform
    Room 119, 745 Bannatyne Avenue
    Basic Medical Science Building
    University of Manitoba (Bannatyne campus)
    Winnipeg, MB R3E 0J9

    204-789-3508