Equipment

Overview of the image analysis station

The image analysis station is an advanced tool designed for researchers who require high-resolution imaging and sophisticated analysis capabilities. With integrated machine learning tools, it simplifies complex tasks like colocalization and 3D deconvolution. The station's user-friendly interface ensures you can easily handle detailed imaging tasks and achieve precise measurements and visualisation, making it a valuable resource for your research.

Key features

Software

• Microsoft Office: For documentation and report generation.
• ZEN Desk 3.1: Provides specialized image analysis functions, perfect for advanced imaging tasks such as colocalization and 3D deconvolution.

Hardware

• Operating system: The station runs on Windows 10 Enterprise (64-bit), ensuring reliable performance for your image analysis tasks.
• Monitor: Equipped with a 22” Samsung monitor, providing a clear and precise display of your images for accurate analysis.

High-resolution analysis

The image analysis station offers advanced tools for processing and analysing images, including:

• Colocalization studies: Helps identify the spatial relationship between different molecular markers in your samples.
• 3D deconvolution: Enhances the clarity and resolution of 3D images by removing blurring effects.
• Extended depth of focus: Produces sharper images of specimens at varying depths, improving the overall quality of your analysis.
• Automated Intellesis machine learning segmentation: Uses machine learning to segment images with high accuracy, improving the reliability of your results.

Measurement tools

The station provides precise measurement functions to help you analyse your samples accurately and reliably. These tools enable you to perform detailed quantification and analysis of specimen characteristics.

Overview of the JEOL JEM-1400 Flash

The JEOL JEM-1400 Flash is a high-resolution transmission electron microscope (TEM) designed for exploring specimens at the atomic level. It is ideal for studying a wide range of samples, from biological tissues to materials and complex structures. The system's support for tomography enables 3D imaging, providing in-depth insights into your samples.

Key features

Software

• AMT V701 software: This software provides full control over the microscope, image acquisition, and data processing. It enhances imaging quality, manages microscope functions, and simplifies data analysis.

Hardware

• Electron optics and LaB6 electron gun: Equipped with advanced electron optics and a LaB6 electron gun, the JEOL JEM-1400 Flash delivers a stable, high-intensity electron beam for exceptional image clarity.
• NANOSPRT43 CMOS CCD camera: This high-resolution camera captures detailed electron images, crucial for structural analysis.

Manual objective and stage control

• Both the objective and stage are manually controlled, giving you precise adjustments for focus, magnification, and sample positioning. This feature provides flexibility for highly targeted imaging.

Imaging capabilities

Magnification range

• The microscope offers a wide range of magnifications, from 50X to 1,500,000X, allowing for both large-scale structural studies and detailed examination of fine structures.

Accelerating voltage settings

• Multiple voltage settings allow you to optimize imaging conditions based on your sample needs:
  • 60kV: Standard voltage for general imaging and delicate samples.
  • 80kV: Provides deeper penetration for thicker specimens.
  • 100kV: Ideal for high-resolution imaging at deeper levels.
  • 120kV: Maximum voltage for atomic-level resolution.

Light source

• The LaB6 electron gun ensures a stable and intense electron beam, delivering superior image quality and enabling high-resolution imaging at fine scales.

Overview of the Leica ARTOS 3D ultramicrotome

The Leica ARTOS 3D ultramicrotome provides precise control for sectioning both biological and material samples. It offers customizable settings for sectioning speeds, cutting angles, and visibility, making it suitable for a wide range of research applications that require high-resolution, accurate sample preparation.

Key features

Adjustable sectioning and return speeds

• The Leica ARTOS 3D allows precise control over the sectioning and return speeds. This flexibility makes it adaptable to different sample types and research requirements.

Customizable cutting angles

• The ultramicrotome features adjustable cutting angles, giving you the ability to tailor the tool for various materials and specific research needs.

Back illumination

• The back illumination improves visibility of the sample during sectioning, helping you monitor the process in real-time and achieve high-quality results.

Knife and specimen rotation

• With knife and specimen rotation capabilities, the Leica ARTOS 3D provides increased precision and flexibility for optimal sample positioning during preparation.

Performance and capabilities

Section thickness range

• The Leica ARTOS 3D supports a section thickness range from 50 to 500 nanometers, allowing you to achieve fine resolution for detailed analysis of your samples.

Serial sectioning capabilities

• This ultramicrotome’s serial sectioning function enables you to make consecutive sections with high precision, which is essential for 3D imaging and detailed structural analysis.

Isolation table

• The isolation table minimizes vibrations and external disturbances, ensuring stable and accurate sectioning to produce the best possible results.

Overview of the Leica EM Ultramicrotome

The Leica EM Ultramicrotome is a highly versatile tool designed for precise sample preparation. With adjustable feed control and high milling speeds, it offers flexibility for both routine and complex sample processing. Equipped with tungsten carbide and diamond milling tools, this ultramicrotome ensures high-quality results for a variety of materials.

Key features

Adjustable feed control

• The Leica EM Ultramicrotome offers step sizes from 0.1 to 100 micrometers, allowing for fine adjustments to meet your specific sample preparation needs.

High milling speeds

• Milling speeds range from 300 to 20,000 rpm, providing fast and efficient processing for a broad range of sample types.

90° corner accuracy

• The ultramicrotome maintains 90° corner accuracy, ensuring precise sample preparation and optimal geometry for further analysis.

Parallel block face

• It keeps a perfectly parallel block face, which is critical for consistent and reproducible results when sectioning samples.

Performance and capabilities

Milling angle range

• With a 60° milling angle range, the Leica EM Ultramicrotome offers flexibility to handle a variety of sample shapes and sizes.

Tungsten carbide and diamond milling tools

• Equipped with tungsten carbide and diamond milling tools, the ultramicrotome ensures superior control and versatility for preparing high-quality samples across a range of materials.

Overview of the Leica UC7

The Leica UC7 ultramicrotome offers customizable cutting processes, providing precise control over sectioning. With adjustable speeds, tunable cutting angles, and an anti-vibration table, this tool ensures stable and high-quality sectioning. The advanced illumination system further enhances visibility, making it suitable for a wide range of research applications.

Key features

Adjustable sectioning and return speeds

• The Leica UC7 allows you to adjust both sectioning and return speeds, offering flexibility for various research needs and ensuring optimal results for different types of samples.

Fine-tuned cutting angles

• The cutting angle can be precisely adjusted, allowing you to tailor the sectioning process to meet the specific requirements of your specimens.

Back illumination

• The back illumination system enhances sample visibility, providing clear views of your specimen, even for very thin samples.

Knife and specimen rotation

• This ultramicrotome includes knife and specimen rotation features, allowing you to adjust the position of the sample for accurate, precise cuts.

Section thickness range

• With a section thickness range from 50 to 500 nm, the Leica UC7 enables detailed microtomy, providing the precision needed for in-depth analysis.

Stability and performance

Integrated anti-vibration table

• The integrated anti-vibration table minimizes external disturbances, ensuring stable, reliable sectioning and reproducible results.

Overview of the Phillips CM10

The Phillips CM10 is a transmission electron microscope (TEM) designed for high-resolution imaging across a range of research fields, including biology, materials science, and nanotechnology. With adjustable voltage settings and a user-friendly interface, it provides detailed images of structural features at the atomic level, making it ideal for accurate, high-quality results.

Key features

Software

• The Phillips CM10 uses AMT 600 software, which allows you to adjust imaging parameters for optimal image quality. This software also simplifies data analysis and ensures high-quality image capture for precise results.

Hardware

• The microscope features an advanced computer system for precise control over voltage and magnification settings.
• Tungsten filament: The electron source provides stable, consistent high-quality electron beams for imaging.

Manual objective and stage control

• Manual objective control: Fine-tune focus and magnification by adjusting the objective lenses for detailed imaging.
• Manual stage control: Position your sample with precision for accurate and consistent imaging.

Accelerating voltage settings

The Phillips CM10 offers several voltage settings to balance resolution with specimen penetration:

• 40kV: Ideal for high-resolution imaging of surface features, especially delicate samples.
• 60kV: Standard voltage for general-purpose imaging, balancing resolution and sample preparation.
• 80kV: Provides deeper penetration, suitable for thicker or denser materials.
• 100kV: Maximum voltage for high-resolution imaging at the nanoscale.

Magnification range

The Phillips CM10 supports a broad magnification range to meet various imaging needs:

• LM 18-530X: Low magnification for viewing larger sample areas and structural features.
• 800X to 92,000X: High magnification for detailed imaging of fine structures, sub-cellular components, or individual molecules and nanoparticles.

Light source

• The tungsten filament serves as a stable and efficient light source, ensuring high-quality, consistent electron beam generation for accurate imaging results.

Camera

• The Advantage HR-B digital CCD camera captures high-resolution black-and-white images, offering exceptional detail for structural and compositional analysis.

Overview of the Reichert OmU3 Ultramicrotome

The Reichert OmU3 Ultramicrotome is a precision tool designed for high-quality sectioning. It provides flexible controls for cutting speeds, angles, and thicknesses. With features like back illumination and rotation capabilities, this ultramicrotome gives you comprehensive control over sample preparation, making it suitable for both biological tissues and materials. It's an essential tool for detailed and accurate investigations.

Key features

Adjustable sectioning and return speeds

• The Reichert OmU3 allows for precise adjustment of both sectioning and return speeds. This flexibility makes it adaptable to different specimen types and research needs, giving you full control over the cutting process.

Customizable cutting angles

• With the customizable cutting angles, you can tailor the cutting process to match the specific requirements of your sample preparation, ensuring optimal results for each specimen.

Back illumination for enhanced visibility

• The back illumination feature improves sample visibility, making it easier to see and section challenging or delicate specimens. This helps you achieve more accurate sectioning for high-quality results.

Knife and specimen rotation

• The knife and specimen rotation capabilities allow for precise adjustments to the cutting orientation, giving you the flexibility needed for accurate sectioning. This enhances the precision and quality of your work.

Section thickness range

• The Reichert OmU3 offers a section thickness range from 50 to 500 nanometers, allowing you to create ultra-thin sections ideal for high-resolution imaging and detailed analysis.

Overview of the Teloview computer

The Teloview computer is a crucial tool for researchers handling complex image data. With its powerful software suite and high-performance hardware, it enables detailed image analysis, including segmentation, quantification, and advanced statistical modeling. Whether you are working with biological, material, or other scientific images, the Teloview computer provides all the necessary tools to extract valuable insights from your data.

Key features

Specialized software

• The Teloview computer runs MatLab, a versatile programming platform for data analysis and visualization. It also includes TeloView, software specifically designed for image analysis. Together, these applications allow you to process large datasets, perform complex computations, and extract meaningful insights from your imaging experiments.

High-performance hardware

• Operating on Windows 10 Ultimate (64-bit), the system provides a stable and efficient environment for data processing. It is equipped with a 22-inch Samsung monitor that delivers high-resolution display quality, ensuring your images and datasets are clear and easy to analyze.

Advanced image analysis capabilities

• The Teloview computer supports advanced image analysis techniques such as image segmentation, quantification, and visualization. It is compatible with a variety of scientific imaging formats and provides the computational power needed for high-level analysis, including statistical modeling. Depending on the dataset, it may also support machine learning applications.

Overview of the Zeiss Imager A2

The Zeiss Imager A2 provides powerful imaging capabilities for researchers needing precise and flexible microscopy. Its advanced features—such as 3D rendering, DIC, and fluorescence imaging—make it suitable for a variety of research applications, including cellular studies and materials science. Whether you're conducting dynamic studies with time-lapse imaging or performing high-resolution fluorescence imaging, this microscope is equipped with the tools necessary for detailed analysis and collaboration.

Key features

Advanced software

• The Zeiss Imager A2 is operated using ZEN 3 Pro software, which simplifies microscope control, image acquisition, and analysis. This software supports multi-dimensional analysis, advanced image processing, and integrates with the microscope's automation features, increasing efficiency for complex imaging tasks.

Versatile hardware

• The system offers both manual and automated controls, providing flexibility depending on your imaging needs.
• Dual co-observer ports enable shared observation, making it suitable for both individual research and collaborative environments.
• Integrated computer monitors allow for real-time analysis, enhancing interactive use.

Imaging capabilities

The Zeiss Imager A2 includes several key imaging features for detailed and precise analysis:

• 3D rendering: Capture multiple focal planes to create detailed 3D images of your sample.
• DIC (Differential interference contrast): Enhance imaging of transparent specimens, revealing fine details like cellular structures.
• Extended depth of focus: Combine multiple focal planes for sharper, more detailed images.
• Fluorescence imaging: Ideal for visualizing fluorescently labeled samples and specific molecules.
• Tiling: Capture overlapping images to create a larger field of view for large specimens (e.g., tissue samples).
• Time-lapse imaging: Record images over time to observe dynamic processes, such as cell movement or growth.
• Z-stack: Build 3D images by stacking images taken at different focal depths for a more detailed, multi-level view.

Objectives

The Zeiss Imager A2 is equipped with several high-quality objectives to meet various magnification needs:

• 2.5X/0.075 Plan-Neofluar: Low magnification for large samples or general imaging.
• 5X/0.16 Plan-Apochromat: Ideal for detailed imaging of larger structures.
• 10X/0.45 Plan-Apochromat: Suitable for intermediate magnification applications.
• 20X/0.8 Plan-Apochromat: High magnification for detailed cellular or tissue imaging.
• 63X/1.4 Oil Plan-Apochromat: Very high magnification with oil immersion for exceptional clarity.

Light source

The Zeiss Imager A2 uses the Colibri light source, which features multiple LEDs for different wavelengths:

• 385nm LED
• 430nm LED
• 475nm LED
• 555nm LED
• 590nm LED
• 630nm LED
• 735nm LED

Camera options

Choose from the following camera options to capture high-quality images:

• AxioCam 105: A color camera for capturing high-quality images with accurate color representation.
• AxioCam MRm: A black-and-white camera that offers high resolution, especially useful for detailed imaging with fluorescence and contrast-enhanced techniques.

Overview of the Zeiss ELRYA PS.1

The Zeiss ELRYA PS.1 is a high-performance microscope tailored for advanced research in molecular biology and cellular imaging. Its super-resolution imaging capabilities make it ideal for studying molecular interactions, fine cellular structures, and processes that require exceptional detail. The ELRYA PS.1 is equipped with powerful hardware and sophisticated software, making it versatile for various applications, including fluorescence imaging, 3D rendering, and co-localization analysis.

Key features

Advanced software

• The Zeiss ELRYA PS.1 is powered by ZEN D software, which provides comprehensive control over microscope operation, image acquisition, and post-processing.
• Key features include:
  • 3D rendering for detailed visualization.
  • Co-localization analysis for studying molecular interactions.
  • Enhanced resolution imaging for high-clarity results in complex molecular and cellular research.

Powerful hardware

• The system includes a laser system and multiple filter options to ensure clarity and precision in fluorescence imaging.
• It comes with an automated stage and objective control for high-resolution imaging, even at higher magnifications.
• Integrated computer monitors provide real-time analysis and control for a seamless research experience.

Objective control

• Automated objective control allows for smooth adjustments in magnification and precise positioning, ensuring high-quality imaging across different levels of detail.
• Easy transitions between objectives make it suitable for both low and high magnification imaging.

Stage control

• The automated stage offers control in the X, Y, and Z axes, enabling precise specimen positioning.
• This supports advanced techniques like Z-stacking to generate 3D images.

Imaging capabilities

The Zeiss ELRYA PS.1 offers several advanced imaging techniques:

• 3D rendering: Visualize depth in specimens by stacking multiple focal planes to create 3D images.
• Co-localization analysis: Study molecular interactions by analyzing the overlap of different fluorescent markers in your sample.
• Fluorescence imaging: Capture detailed images of fluorescently labeled samples to track molecules or structures precisely.
• Photoactivated Localization Microscopy (PALM): Achieve super-resolution imaging by pinpointing individual fluorescent molecules.
• Structured Illumination Microscopy (SIM): Improve image resolution beyond the diffraction limit using patterned light.
• Z-stack imaging: Capture images at multiple focal depths and combine them into a 3D representation for detailed analysis.

Objectives

The Zeiss ELRYA PS.1 comes equipped with a selection of high-quality objectives for various magnification needs:

• Zeiss Alpha Plan-Apochromat 100X/1.46 Oil DIC Elyra: High-resolution objective for super-resolution imaging.
• Zeiss Alpha Plan-Apochromat 100X/1.57 Oil DIC Korr: Enhanced oil immersion objective for improved contrast and resolution.
• Zeiss N-Achroplan 10X/1.025: General-purpose low magnification objective for wide-field imaging.
• Zeiss Plan-Apochromat 20X/1.08: Mid-range magnification offering a balance of resolution and field of view.
• Zeiss Plan-Apochromat 40X/1.4 Oil DIC: High magnification for detailed cellular or tissue imaging with oil immersion.
• Zeiss Plan-Apochromat 63X/1.40 Oil DIC: Very high resolution for imaging small structures with exceptional clarity.

Light source

The Zeiss ELRYA PS.1 uses a mercury bulb for broad-spectrum illumination, along with several lasers to excite specific fluorophores for precise fluorescence imaging:

• HR Diode 405 nm (blue, DAPI)
• HR Diode 488 nm (green)
• HR DPSS 561 nm (red)
• HR Diode 642 nm (far red)

These lasers allow for detailed excitation of fluorescent markers, ensuring high-quality imaging across different sample types.

Filters

The system includes two filter wheels for optimal fluorescence imaging:

• Filter Wheel 1: Filters for wavelengths 420-480 nm, 570-640 nm, and other ranges to control excitation and emission.
• Filter Wheel 2: Filters for 495-550 nm and 570-620 nm to further enhance fluorescence signal control and imaging precision.

Camera

The Zeiss ELRYA PS.1 is equipped with a high-resolution black-and-white camera, essential for super-resolution and fluorescence imaging. This camera captures fine details with exceptional clarity, making it ideal for imaging at both high magnification and with fluorescence markers.

Overview of the Zeiss Lumar V.12

The Zeiss Lumar V.12 is an advanced imaging system designed for researchers who need flexibility between brightfield and fluorescence modes. It allows you to view specimens in natural light for routine observations or in fluorescence for studying specific molecular interactions. Additionally, its ability to generate 3D images and capture time-lapse sequences makes it essential for dynamic biological research, including the study of cellular processes, chemical reactions, and molecular behavior.

Key features

Software

• ZEN 2 software provides full control over image acquisition, processing, and analysis.
• It supports multiple imaging modes, including brightfield, fluorescence, and time-lapse imaging, making it versatile for various research applications.
• Z-stack analysis allows the creation of 3D images, ideal for exploring complex sample structures at various depths.

Hardware

• Integrated computer monitors with a user-friendly interface enable efficient control and analysis.
• The Zeiss Lumar V.12 is designed for both manual and automated operations, allowing you to customize your workflow to suit your research needs.
• Whether you're a beginner or an experienced user, the system simplifies capturing detailed images and managing data.

Objective control

• Manual objective lenses can be easily swapped to accommodate different imaging needs.
• This flexibility provides precise control over magnification and resolution, ensuring high-quality results for every sample.

Stage control

• The rotating stage with X and Y-axis movement allows excellent flexibility in positioning your sample.
• This feature is particularly useful for accurate imaging and detailed observation of different areas of the specimen.

Imaging capabilities

Brightfield imaging

• Ideal for observing stained or naturally colored specimens under normal light.
• Perfect for routine examinations and general observations.

Extended depth of focus

• Enhances the clarity of images across multiple focal planes, providing sharp visuals even with samples that have significant topographical variation.

Fluorescence imaging

• Observe fluorescently labeled samples to study cellular components, molecular interactions, and specific biomarkers.

Time-lapse imaging

• Capture changes over time to study dynamic biological processes, such as cellular movement or chemical reactions.
• Visualize the process over an extended period for in-depth analysis.

Z-stack imaging

• Capture images from multiple focal planes and combine them into 3D representations of your sample.
• This helps explore the specimen’s structure in greater depth, ideal for studying complex samples.

Objectives

The Zeiss Lumar V.12 includes several objectives for varying magnification needs:

• Zeiss 0.8X 80mm: Low magnification with a long working distance, ideal for large samples and wide field of view.
• Zeiss 1.2X 47mm: Offers a balance between field of view and depth with a medium working distance.
• Zeiss 1.5X 30mm: Low magnification with a short working distance, providing higher resolution for detailed views.

Light source

• The Zeiss Lumar V.12 uses an HBO 100 mercury bulb as its primary light source.
• This provides bright and stable illumination for both brightfield and fluorescence imaging, ensuring consistent lighting across all modes.

Filters

The system includes several filter sets for optimizing fluorescence imaging:

• Zeiss Filter Set 20
• Zeiss Filter Set 38HE
• Zeiss Filter Set 43HE
• Zeiss Filter Set 49

Cameras

The Zeiss Lumar V.12 offers two camera options:

• AxioCam HRm: A black-and-white camera optimized for high-contrast imaging, perfect for detailed analysis in both brightfield and fluorescence modes.
• AxioCam ICc 3: A color camera designed to capture detailed color images, ideal for specimens requiring color differentiation and in-depth observation.

Overview of the Zeiss Lumar V.12

The Zeiss Lumar V.12 is a powerful imaging system designed for viewing and analyzing complex, three-dimensional specimens. It supports both natural light and fluorescence imaging, making it ideal for studying structural details and observing dynamic changes in samples over time. The system is versatile and useful for a wide range of applications, including molecular biology, histology, and material science.

Key features

Software

• The AxioVision software provides robust tools for capturing, processing, and analyzing brightfield and fluorescence images.
• Full control over the system allows for flexibility in various imaging tasks, making it suitable for different research applications.

Objective control

• The microscope features manual objective lens exchange, which allows you to select lenses based on your specimen's magnification and working distance.
• This provides precise control over the level of detail you want in your images.

Stage control

• The rotating stage moves along both X and Y axes, allowing for easy and precise specimen positioning.
• This feature helps capture images from multiple angles, ensuring optimal imaging conditions.

Capabilities

Extended depth of focus

• Combines multiple focal planes into a single image, offering clearer and more detailed views of three-dimensional specimens.
• Particularly useful for imaging large or complex samples that require depth clarity.

Fluorescence imaging

• The fluorescence imaging feature enables you to observe fluorescently labeled samples.
• This is essential for studying specific structures or molecular interactions in molecular biology, histology, and material science.

Time-lapse imaging

• Time-lapse imaging captures a series of images over time, allowing you to study dynamic changes, such as cellular movement or chemical reactions.

Z-stack imaging

• Z-stack imaging allows you to capture images at different focal depths and combine them to create three-dimensional images.
• This is ideal for visualizing complex structures that cannot be captured in a single focal plane.

Objectives

The Zeiss Lumar V.12 includes various objective lenses designed for different magnifications and working distances:

• Zeiss 0.8X 80mm: Low magnification with a long working distance, ideal for general imaging of large specimens.
• Zeiss 1.2X 47mm: Low magnification with a medium working distance, useful for observing larger structures with moderate detail.
• Zeiss 1.5X 30mm: Low magnification with a short working distance, perfect for detailed views of smaller structures.

Light source

• The system uses an HBO 100 mercury bulb as its light source, providing stable, bright illumination for both brightfield and fluorescence imaging.

Filters

The Zeiss Lumar V.12 includes several Zeiss filter sets optimized for fluorescence imaging:

• Zeiss Filter Set 49
• Zeiss Filter Set 20HE
• Zeiss Filter Set 38HE

These filter sets are designed to enhance fluorescence imaging by allowing observation of specific wavelengths that are necessary for your research.

Cameras

The system offers two camera options for capturing images:

• AxioCam HRm: A black-and-white camera designed for high-contrast imaging in both fluorescence and brightfield modes. Ideal for detailed specimen analysis.
• AxioCam ICc 3: A color camera that captures detailed color images, perfect for specimens that require accurate color differentiation.

Overview of the Zeiss Imager M2

The Zeiss Imager M2 is a versatile imaging system designed for researchers studying both naturally coloured and fluorescently labelled specimens. With advanced software and precise control options, it supports comprehensive image analysis and quantification, making it suitable for a wide range of research applications, from molecular biology to material science.

Key features

Software

The ZEN 3 Pro software powers the Zeiss Imager M2, offering a full range of tools for controlling the microscope, acquiring images, and conducting detailed analysis and quantification. ZEN 3 Pro ensures precision and ease of use, helping researchers fully harness the microscope’s capabilities.

Objective control

The Zeiss Imager M2 offers both manual and automated objective control, giving you the flexibility to switch between objectives or use automated settings. This feature enhances precision and simplifies the imaging process.

Stage control

The microscope is equipped with manual and rotating stage control, enabling precise movement along the X and Y axes. This allows for accurate sample positioning and imaging from different angles.

Capabilities

Analysis

The Zeiss Imager M2 includes built-in tools for analyzing and quantifying image data. You can quickly obtain detailed metrics and visualize your results, making it ideal for various research applications.

Brightfield imaging

For traditional imaging, the Zeiss Imager M2 offers high-quality brightfield imaging of stained or naturally coloured specimens. This provides sharp, clear images, making it ideal for detailed examination.

Co-localization analysis

Co-localization analysis enables the study of the overlap between different fluorescent markers. This feature is essential for researching molecular interactions and identifying specific regions of interest within samples.

Fluorescence imaging

The Zeiss Imager M2 supports fluorescence imaging, allowing you to observe fluorescently labelled samples. This is crucial for visualizing specific structures, proteins, or molecular markers, especially in studies related to cell biology, histology, and molecular science.

Objectives

The microscope comes with several Zeiss objective lenses for varying magnifications and working distances:

• Zeiss 2.5X/0.075 ∞/0.17 (EC Plan-Neofluar): Low magnification, wide field of view, perfect for scanning large specimens.
• Zeiss 5X/0.16 Ph1 ∞/0.17 (EC Plan-Neofluar): Low magnification with phase contrast for viewing larger structures in more detail.
• Zeiss 10X/0.3 Ph1 ∞/- (EC Plan-Neofluar): Medium magnification with phase contrast, ideal for examining cell structures.
• Zeiss 20X/0.4 Ph1 Ph2-Korr ∞/0-1.5 (LD Plan-Neofluar): Medium magnification with phase contrast correction, perfect for detailed cellular imaging.
• Zeiss 40X/0.6 Ph1 Ph2-Korr ∞/0-1.5 (LD Plan-Neofluar): High magnification with phase contrast, great for detailed cellular examination.
• Zeiss 100X/1.3 oil Ph3 ∞/0.17 (EC Plan-Neofluar): High magnification, oil immersion lens for high-resolution imaging of fine details.

Light source

The Zeiss Imager M2 is equipped with the Colibri LED light source, which includes modules with multiple wavelengths, supporting a variety of fluorescence imaging applications:

• 365nm LED
• 470nm LED
• 555nm LED
• 590nm LED

Filters

The system includes several Zeiss filter sets, optimized for fluorescence imaging:

• Zeiss Filter Set 38HE
• Zeiss Filter Set 43HE
• Zeiss Filter Set 49HE
• Zeiss Filter Set 50HE
• Zeiss Filter Set 62HE

These filters enhance the system’s ability to capture fluorescence at specific wavelengths for detailed analysis.

Differential interference contrast (DIC)

The Zeiss Imager M2 includes DIC (Differential Interference Contrast) technology, which enhances contrast in transparent samples without the need for staining. This provides clearer, more detailed images, making it ideal for live or unstained specimens.

Cameras

The system offers two camera options for capturing high-quality images:

• AxioCam HRc: A colour camera designed for high-resolution imaging with excellent colour accuracy. It’s ideal for both brightfield and fluorescence imaging.
• AxioCam MRm: A black-and-white camera designed for high-contrast, high-resolution imaging of fluorescent specimens. Perfect for detailed fluorescence studies.

Overview of the The Zeiss Imager M2 Scan 

The Zeiss Imager M2 Scan is a versatile microscope for high-resolution imaging of stained and fluorescently labeled specimens. It supports brightfield, fluorescence, and phase contrast imaging, ideal for both general and detailed analysis. Key features include tiling, time series, and Z-stack for dynamic and 3D imaging. Powered by ZEN 3.8 Pro software, it offers advanced analysis tools, and its manual/automated controls ensure precise adjustments. With various objective lenses and LED light sources, it’s well-suited for detailed cellular imaging in research.

Key features

Software

The system is equipped with ZEN 3.8 Pro software, providing powerful tools for image analysis, visualization, and quantification.

Objective control

The Zeiss Imager M2 Scan offers both manual and automated objective control, giving users flexibility and precision in adjusting magnification to meet experimental needs.

Stage control

The system features manual and automated stage control across the X, Y, and Z axes, ensuring accurate, repeatable positioning of samples for complex imaging.

Capabilities

Brightfield

This mode allows for viewing stained or naturally colored specimens, ideal for general imaging of traditional samples.

Fluorescence

The system enables observation of fluorescently labeled samples, perfect for tracking specific molecules, proteins, or structures.

Phase contrast

Phase contrast imaging enhances the contrast of transparent specimens without the need for staining, making it ideal for live cell imaging.

Tiling

The system can create large composite images by stitching together multiple fields of view, useful for imaging larger or complex specimens.

Time series

With time series imaging, you can capture a sequence of images over time, allowing for the study of dynamic changes in living specimens.

Z-stack

Create three-dimensional images by stacking images from different focal planes, providing detailed 3D visualizations of cellular structures.

Objectives

The Zeiss Imager M2 Scan offers a variety of objective lenses for different magnification needs:

• Zeiss 2.5X/0.085 ∞/0.17 – Plan-Neofluar: Low magnification with a high depth of field, ideal for wide-field overview imaging.
• Zeiss 5X/0.16 ∞/0.17 – Plan-Apochromat: Low magnification with improved color correction, perfect for high-quality imaging.
• Zeiss 10X/0.45 ∞/0.17 – Plan-Apochromat: Intermediate magnification with enhanced color correction, suitable for detailed specimen imaging.
• Zeiss 20X/0.08 ∞/0.17/OFN25 – Plan-Apochromat: High magnification optimized for fluorescence and wide-field imaging.
• Zeiss 63X/1.4 Oil ∞/0.17 – Plan-Apochromat: High magnification with oil immersion, providing excellent resolution for fine structural details.

Light source

The Zeiss Imager M2 Scan includes multiple LED light sources for fluorescence imaging:

• 385nm LED
• 430nm LED
• 475nm LED
• 555nm LED
• 590nm LED
• 630nm LED
• 735nm LED

Filters

The system is equipped with the following Zeiss filter sets for fluorescence imaging:

• Zeiss Filter Set 38HE
• Zeiss Filter Set 49HE
• Zeiss Filter Set 63HE
• Zeiss Filter Set 112

Cameras

The system includes two camera options for imaging:

• AxioCam 305: Color camera for detailed color imaging of samples.
• AxioCam 705: Black-and-white camera for high-contrast imaging, ideal for clear visualization of cellular structures.

Overview of the Zeiss Observer D1

The Zeiss Observer D1 is a high-quality imaging system designed for live cell analysis. It combines advanced imaging capabilities with ease of use, making it ideal for studying live cell dynamics. With its time-lapse imaging features and ability to capture detailed cellular structures, this system supports a broad range of cell biology applications.

Key features

Software

The ZEN 3 Pro software powers the Zeiss Observer D1, offering robust tools for live cell imaging, analysis, and visualization. It ensures precise control over your imaging experiments, making it easier to capture high-quality data.

Objective control

The system provides manual objective control, allowing for precise imaging and easy adjustment between objectives. This flexibility ensures the ability to tailor your imaging setup to specific needs.

Stage control

The Zeiss Observer D1 is equipped with automated stage control for accurate sample movement across the field of view with minimal intervention. This feature allows you to focus on data collection without constantly adjusting the stage.

Capabilities

Brightfield imaging

The system provides brightfield imaging, offering clear images of stained or naturally coloured specimens. It’s ideal for studying cell morphology and general cell observations.

Fluorescence imaging

With fluorescence imaging, you can observe fluorescently labelled samples, making it perfect for tracking specific proteins or molecules in your research.

Phase contrast

Phase contrast enhances contrast in transparent specimens, making it ideal for live cell imaging without the need for staining. This feature allows for the visualization of live cells in their natural state.

Tiling

The tiling function creates large composite images from multiple fields of view. This is useful for imaging larger samples or areas that require a detailed overview.

Time series

The time series feature captures a sequence of images over time, allowing you to study dynamic changes in living cells, such as cell movement or interactions.

Z-stack

The Z-stack function builds 3D images by stacking images from multiple focal planes. This allows for detailed visualization of complex cell structures in three dimensions.

Objectives

The Zeiss Observer D1 comes with several Zeiss objective lenses, designed for varying magnifications and working distances:

• Zeiss 5X/0.16 Ph1 ∞/0.17 (EC Plan-Neofluar): Low magnification with phase contrast, ideal for scanning large cell cultures or tissues.
• Zeiss 10X/0.3 Ph1 ∞/- (EC Plan-Neofluar): Intermediate magnification with phase contrast, useful for examining cellular details.
• Zeiss 20X/0.4 Ph1 Ph2-Korr ∞/0-1.5 (LD Plan-Neofluar): High magnification with phase contrast and long working distance, perfect for detailed live cell imaging.
• Zeiss 40X/0.6 Ph1 Ph2-Korr ∞/0-1.5 (LD Plan-Neofluar): High magnification with phase contrast and long working distance, ideal for fine cellular details.

Light source

The Colibri LED light source provides excellent support for fluorescence imaging, with multiple LED modules offering various wavelengths for optimal sample visualization:

• 365nm LED
• 470nm LED
• 555nm LED
• 590nm LED

Filters

The Zeiss Observer D1 includes several Zeiss filter sets optimized for fluorescence imaging:

• Zeiss Filter Set 02
• Zeiss Filter Set 10
• Zeiss Filter Set 20
• Zeiss Filter Set 43HE
• Zeiss Filter Set 62HE

These filters enable efficient capture of fluorescence at specific wavelengths.

Camera

The system is equipped with the AxioCam MRm, a black-and-white camera designed for high-contrast, high-resolution imaging. It’s perfect for capturing detailed live cell images, especially for fluorescence imaging.

Overview of the Zeiss Observer 7

The Zeiss Observer 7 is a versatile tool for live cell imaging, providing superior resolution and precise control over imaging conditions. With capabilities like time-lapse imaging and 3D reconstructions of cells, this system is well-suited for in-depth studies of cellular dynamics. Its integrated environmental controls ensure optimal conditions for your samples, delivering reliable results for your research.

Key features

Software

Operated by ZEN 3 Pro software, this system offers advanced tools for live cell imaging, analysis, and visualization, giving you full control over your experiments and data collection.

Objective control

The automated objective control allows seamless switching between objectives with high precision. This feature helps you obtain clear, detailed images with minimal effort.

Stage control

The automated stage control enables precise, programmable sample movements, allowing you to conduct dynamic studies with minimal intervention and improved reproducibility.

Capabilities

Brightfield imaging

The system provides brightfield imaging, perfect for observing stained or naturally coloured specimens. This feature delivers clear contrast and is ideal for cellular morphology studies.

Fluorescence imaging

With fluorescence imaging, you can visualize fluorescently labelled samples. This capability is perfect for tracking specific molecules or proteins in living cells.

Phase contrast

Phase contrast enhances contrast in transparent specimens, making it a perfect choice for live cell imaging without the need for staining.

Tiling

The tiling function stitches together multiple fields of view to create large composite images. This is particularly useful for imaging larger areas or samples that exceed the typical field of view.

Time series

The time series feature allows you to capture a sequence of images over time, enabling you to observe dynamic changes in living cells, such as movement or interactions.

Z-stack

With Z-stack imaging, you can build 3D images from multiple focal planes. This provides a complete view of cellular structures and enhances your ability to analyze complex specimens.

Environmental controls

The Zeiss Observer 7 is equipped with integrated environmental controls to maintain optimal conditions for live cell cultures during imaging:

• CO2: Regulates carbon dioxide levels, ensuring an ideal environment for live cell cultures.
• Humidity: Monitors and adjusts humidity levels to maintain stable conditions for your samples.
• Temperature: Controls temperature to prevent stress on live cell cultures during imaging.

Objectives

The system features several Zeiss objectives designed for different magnifications and imaging needs:

• Zeiss 5X/0.16 Ph1 ∞/0.17 (EC Plan-Neofluar): Low magnification with phase contrast, ideal for scanning large specimens.
• Zeiss 10X/0.3 Ph1 ∞/- (EC Plan-Neofluar): Intermediate magnification with phase contrast, perfect for detailed cell observations.
• Zeiss 20X/0.4 Ph1 Ph2-Korr ∞/0-1.5 (LD Plan-Neofluar): High magnification with phase contrast and a long working distance, ideal for live cell imaging.
• Zeiss 40X/0.6 Ph1 Ph2-Korr ∞/0-1.5 (LD Plan-Neofluar): High magnification with phase contrast and long working distance, perfect for fine cellular details.
• Zeiss 40X/1.24 Imm Korr DIC M27 (Plan-Apochromat): High magnification with oil immersion and differential interference contrast (DIC) for high-contrast, detailed images of living cells.

Light source

The Colibri LED light source includes multiple wavelength options for optimal fluorescence imaging:

• 365nm LED
• 470nm LED
• 555nm LED
• 590nm LED

These options provide flexibility for a variety of fluorescence imaging applications.

Filters

The system includes Zeiss filter sets specifically designed for fluorescence imaging:

• Zeiss Filter Set 02
• Zeiss Filter Set 10
• Zeiss Filter Set 20
• Zeiss Filter Set 43HE
• Zeiss Filter Set 62HE

These filters enable the use of specific wavelengths for precise fluorescence capture.

Camera

The AxioCam MRm is a black-and-white camera optimized for high-contrast, high-resolution imaging. It is ideal for capturing fine cellular details in both brightfield and fluorescence imaging modes.

The Zeiss Imager Z1 offers advanced imaging and analysis capabilities, making it an excellent tool for exploring intricate cellular structures and dynamic processes. With its high-resolution imaging, 3D visualisation, and sophisticated software, this system is ideal for researchers who need to examine the fine details of their samples with accuracy.

Key features

• Software: Powered by ZEN 3 Pro software, the microscope provides powerful image analysis, visualisation, and data quantification tools to give you in-depth insights into your samples.
• Objective control: The Zeiss Imager Z1 supports both manual and automated objective control, offering flexibility and precision in adjusting magnification to suit your experiment's needs.
• Stage control: The system features both manual and automated stage control across the X, Y, and Z axes, ensuring accurate, repeatable positioning of samples for complex 3D imaging.

Capabilities

• 3D rendering: Combine multiple focal planes to create 3D images, allowing for detailed structural analysis of your samples.
• Deconvolution: Improve image clarity by reducing optical distortions, resulting in enhanced resolution and more detailed images.
• Differential interference contrast (DIC): Boost contrast for transparent specimens, enabling high-detail imaging without the need for staining.
• Fluorescence: Visualise fluorescently labelled samples, perfect for tracking specific molecules or proteins in both living and fixed samples.
• Image analysis: Equipped with powerful analysis tools, enabling you to extract valuable insights from your images.
• Microarray: Analyse multiple samples on a single slide, facilitating high-throughput capabilities for large-scale studies.
• Mosaic: Combine multiple images to create a large, detailed view of a sample, ideal for imaging larger specimens.
• Positions: Set and recall specific sample positions to ensure consistent and reproducible imaging, making it ideal for multi-sample or repetitive imaging.
• Tiling: Capture overlapping images to cover larger areas or fields, useful for imaging more extensive samples.
• Time lapse: Record a series of images over time to capture dynamic changes in living samples.
• Z-stack: Create 3D images by stacking images from different depths, perfect for viewing intricate cellular structures in 3D.

Objectives

• Zeiss 10X/0.3 ∞/- (Plan-Neofluar): Low magnification, general-purpose objective ideal for overview imaging.
• Zeiss 20X/0.8 ∞/0.17 (Plan-Neofluar): Medium magnification, high-resolution lens for detailed specimen imaging.
• Zeiss 40X/0.75 ∞/0.17 (EC Plan-Neofluar): High magnification with enhanced contrast, perfect for cellular observations.
• Zeiss 40X/1.3 oil DIC ∞/0.17 (EC Plan-Neofluar): High magnification with oil immersion and DIC for precise imaging of fine cellular details.
• Zeiss 63X/1.4 oil DIC ∞/0.17 (Plan-Neofluar): Very high magnification with oil immersion and DIC for ultra-high-detail imaging, ideal for fine structural analysis.

Light source

The Colibri LED light source offers multiple wavelength options for fluorescence imaging:

• 365 nm LED
• 470 nm LED
• 555 nm LED
• 590 nm LED

Filters

The system includes Zeiss filter sets for fluorescence imaging:

• Zeiss Filter Set 38HE
• Zeiss Filter Set 43HE
• Zeiss Filter Set 49HE
• Zeiss Filter Set 50HE
• Zeiss Filter Set 62HE

Cameras

• AxioCam 105: A colour camera designed for high-resolution imaging, capturing detailed, full-colour images of your samples.
• AxioCam MRm: A black-and-white camera for high-contrast, high-resolution imaging, perfect for clear, detailed visualisation of cellular structures.

Overview of the Zeiss Imager Z2

The Zeiss Imager Z2 offers advanced imaging capabilities, combining high resolution with features like 3D rendering, extended depth of focus, and precise stage control. This microscope is designed for researchers tracking cellular components or studying dynamic processes, providing the clarity and precision needed for in-depth research.

Key features

Software

Powered by ZEN 3 Pro software, the Zeiss Imager Z2 provides advanced tools for image acquisition, analysis, and visualisation. This software is ideal for research requiring precise imaging and data handling.

Objective control

The microscope offers both manual and automated control for flexible, accurate magnification adjustments, ensuring ease of use across a range of research applications.

Stage control

With both manual and automated control across the X, Y, and Z axes, this system ensures accurate and repeatable sample positioning. This is crucial for complex imaging workflows where precise movement is needed.

Capabilities

3D rendering

The system combines multiple focal planes to create three-dimensional images, offering detailed views of specimens for a more comprehensive analysis.

Differential interference contrast (DIC)

DIC imaging enhances contrast in transparent specimens without the need for staining, allowing high-detail imaging of live or delicate samples.

Extended depth of focus

This feature merges multiple focal planes into one image, improving clarity and sharpness for structures at various depths, which is especially useful for complex, 3D specimens.

Fluorescence imaging

The system enables fluorescence imaging, allowing you to observe fluorescently labelled samples. This feature is perfect for tracking specific cellular components or molecules over time.

Tiling

With the tiling feature, the system captures overlapping images and stitches them together to create a large, detailed composite. This is ideal for imaging larger samples or areas of interest.

Time lapse

Time lapse imaging records a series of images over time, making it easy to study dynamic changes in living samples, such as cell division or cell movement.

Z-stack

The Z-stack feature creates 3D images by stacking multiple focal depths. This allows for enhanced clarity when imaging complex structures at different focal planes.

Objectives

The Zeiss Imager Z2 is equipped with a range of Zeiss objectives for different magnifications and imaging needs:

• Zeiss 2.5X/0.75 ∞/0.17 – EC Plan-Neofluar: Low magnification, wide field of view, suitable for large area imaging.
• Zeiss 5X/0.16 ∞/0.17 – EC Plan-Neofluar: Low magnification, general-purpose lens for overview imaging.
• Zeiss 10X/0.3 ∞/0.17 – EC Plan-Neofluar: Medium magnification, high-resolution lens for detailed specimen imaging.
• Zeiss 20X/0.5 ∞/0.17 – EC Plan-Neofluar: High magnification for enhanced contrast and more detailed specimen imaging.
• Zeiss 40X/0.75 ∞/0.17 – EC Plan-Neofluar: High magnification for enhanced detail and high-contrast images.
• Zeiss 63X/1.4 oil DIC ∞/0.17 – Plan-Neofluar: Very high magnification with oil immersion and DIC for imaging fine structures and high-contrast details.

Light source

The Colibri LED light source offers multiple wavelength options for fluorescence imaging:

• 385 nm LED
• 430 nm LED
• 475 nm LED
• 555 nm LED
• 590 nm LED
• 630 nm LED
• 735 nm LED

These wavelength options provide flexibility for various fluorescence applications.

Filters

The Zeiss Imager Z2 includes a variety of Zeiss filter sets for enhanced fluorescence imaging:

• Zeiss Filter Set 20HE
• Zeiss Filter Set 38HE
• Zeiss Filter Set 43
• Zeiss Filter Set 49
• Zeiss Filter Set 50
• Zeiss Filter Set 62HE
• Zeiss Filter Set 112

These filter sets allow for specific wavelength selection to optimize fluorescence capture.

Cameras

The system includes two camera options:

• AxioCam 208: A colour camera designed for high-resolution imaging and capturing detailed colour images of your samples.
• AxioCam MRm: A black-and-white camera for high-contrast imaging, perfect for capturing detailed visualisation of structures.