The University of Manitoba campuses are located on original lands of Anishinaabeg, Ininew, Anisininew, Dakota and Dene peoples, and on the National Homeland of the Red River Métis. More
University of Manitoba
Winnipeg, Manitoba Canada, R3T 2N2
Use the links below to jump to the section you're looking for.
Optical configuration is available for our flow cytometry instruments on the popular flow cytometry panel design website FluoroFinder.
View optical configuration of all the flow cytometers and designing multi color panels based on the configuration of any one of these flow cytometers. You also have the option to print out the configuration of your favourite instrument as a poster.
Select the markers of your choice (including viability dyes and fluorescence proteins) based on criteria such as targeting species, host species, isotope, clone, antigen density for multicolor panel design.
View the commercially available fluorochromes conjugates for the specific marker of your choice and corresponding fluorochrome intensity. You will also see detailed info of these available antibodies like companies, catalog number, citations, size and price.
Check the spectral overlap of the selected antibody combination for your panel based on optical configuration of our flow cytometry instruments.
This powerful instrument, capable of detecting up to 19 parameters and 16 colours simultaneously, is ideal for both clinical and research applications.
Room 466, Apotex Centre
University of Manitoba (Bannatyne campus)
Its Avalanche photodiode detectors provide great sensitivity and resolution across the entire visible spectrum.
It is equipped with both semi-automatic single tube loader and 96 well plate, giving the researchers more flexibility in sample preparation with reduced hands-on time and better results reliability.
Its unique violet side scatter allows for nanoparticle and microparticle resolutions under 200nm.
The volumetric measurement with continuous flow of this instrument makes it possible for particle enumeration without the needs for counting beads.
CytExpert
| Laser | Mirror/filter | Detection range | Flueorchrome | |
|---|---|---|---|---|
|
1 |
Violet (405nm) |
450/50 BP |
425-475 |
Pacific blue, Dapi, V450, BV421, eFluor450 |
|
2 |
525/40 BP |
505-565 |
AmCyan, V500, BV510 |
|
|
3 |
610/20 BP |
600-620 |
BV605 |
|
|
4 |
660/20 BP |
650-670 |
BV650 |
|
|
5 |
763/42 BP |
742-784 |
BV785 (*BV711 avalilable by request) |
|
|
6 |
Blue (488nm) |
525/40 BP |
505-545 |
FITC, Alexa488, CFSE, GFP |
|
7 |
610/20 BP |
600-620 |
PE-texas red, PE-CF594, propidium iodide |
|
|
8 |
690/50 BP |
665-715 |
PerCP/PerCP-Cy5.5 |
|
|
9 |
Yellow/green (561nm) |
585/42 BP |
564-606 |
PE, dsRed, PI |
|
10 |
610/20 BP |
600-620 |
mCherry, ECD, PE-texas red, PI, PE-CF594 |
|
|
11 |
675/30 BP |
660-690 |
PE-Cy5, PerCP |
|
|
12 |
710/50 BP |
685-735 |
PE-Cy5.5, PerCP-Cy5.5 |
|
|
13 |
763/42 BP |
742-784 |
PE-Cy7, PE-Alexa750 |
|
|
14 |
Red (638nm) |
660/20 BP |
650-670 |
APC, Alexa647, Cy5 |
|
15 |
712/25 BP |
700-724 |
Alexa700 |
|
|
16 |
763/42 BP |
742-784 |
APC-cy7, APC-H7, APC-eFluor780 |
How to read bandpass filter detection range: e.g., 450/50 = 450nm ± (50/2)nm = 450nm ± 25nm = from 425nm to 475nm
This simple yet powerful microscope can allow you to quickly check the protein expression in cells when cells are still in tissue culture vessels, which means the cells will stay in viable sterile state and can go back to incubator after imaging.
It is also capable of multi-colour image acquisition in many different viewing system such as chamber slides, regular glass slides, multi-well tissue culture plates or tissue culture dishes with different diameters.
Room 466, Apotex Centre
University of Manitoba (Bannatyne campus)
Light Source:
Objectives:
Stage Accommodation:
Channels:
This powerful instrument, capable of detecting up to 17 parameters and 15 colors simultaneously, makes it possible to identify and isolate even the rarest cell population you are interested in.
Room 462, Apotex Centre
University of Manitoba (Bannatyne campus)
Samples can be sorted at single cell-basis with sample agitation and temperature control into two or four tubes (15mL centrifuge tube to 1.0mL microtubes), multi-well plates or microscope slides.
It is equipped with Aerosol Management Option (AMO), a device that traps aerosolized particles.
Our Aria-III system is housed in a Class-II Type A2 bio-safety cabinet to ensure that samples remain sterile during sorting process.
It is capable of accommodating most cell types with its 70, 85 and 100 micron nozzles.
20mW 488nm solid state blue laser
17mW 633nm HeNe red laser
30mW 405nm solid state violet laser
1-6. Six fluorescent detectors off 488nm
7-9. Three fluorescent detectors off 633nm
10-15. Six fluorescent parameters off 405nm
16. Forward scatter
17. Side scatter
FACSDiva
| Laser | Mirror/filter | Detection range | Fluorochrome | |
|---|---|---|---|---|
| 1 | Blue (488nm) | 502LP, 530/30 | 515-545 | FITC, GFP, Alexa488 |
| 2 | 556LP, 585/42 | 564-606 | PE, dsRed | |
| 3 | 610LP, 616/23 | 610-627 | PE-Texas Red, PI (live) | |
| 4 | 640LP, 670/30 | 655-685 | PerCP, PE-Cy5, 7-AAD | |
| 5 | 685LP, 695/40 | 685-715 | PE-Cy5.5, PerCP-Cy5.5 | |
| 6 | 735LP, 780/60 | 750-810 | PE-Cy7, PE-Alexa750 | |
| 7 | Red (633nm) | N/A, 660/20 | 650-670 | APC, Alexa647, Cy5 |
| 8 | 690LP, 730/45 | 708-752 | Alexa700 | |
| 9 | 735LP, 780/60 | 750-810 | APC-cy7, APC-H7 | |
| 10 | Violet (405nm) | N/A, 450/40 | 430-470 | Pacific blue, Dapi, V450, BV421 |
| 11 | 502LP, 510/50 | 502-545 | AmCyan, CFP, V500, BV510 | |
| 12 | 545LP, 560/20 | 550-570 | BrilliantViolet570 | |
| 13 | 595LP, 610/20 | 578-592 | BrilliantViolet605 | |
| 14 | 630LP, 660/20 | 650-670 | BrilliantViolet650 | |
| 15 | 690LP, 710/50 | 685-735 | BrilliantViolet711 |
How to read longpass filter detection range
e.g., 502LP—wavelength Longer than 502nm will be allowed to Pass through
How to read bandpass filter detection range
e.g., 530/30 = 530nm ± (30/2)nm = 530nm ± 15nm = from 515nm to 545nm
This powerful instrument, capable of detecting up to 10 parameters and eight colours simultaneously, is ideal for both clinical and research applications. Its newly designed loader carousel allows for automatic loading of up to 40 12x75mm tubes without operator intervention, reducing hands-on time and improving results reliability.
Location:
Room 466, Apotex Centre
University of Manitoba (Bannatyne Campus)
| Laser | Mirror/filter | Detection range | Fluorochrome | |
|---|---|---|---|---|
|
1 |
Blue (488nm) |
502LP, 530/30 |
515-545 |
FITC, GFP, Alexa488 |
|
2 |
556LP, 585/42 |
564-606 |
PE, dsRed |
|
|
3 |
655LP, 670LP |
≥670 |
PerCP, PE-Cy5.5, PI, 7-AAD |
|
|
4 |
735LP, 780/60 |
750-810 |
PE-Cy7 |
|
|
5 |
Red (633nm) |
660/20 |
650-670 |
APC, Alexa647, Cy5 |
|
6 |
735LP, 780/60 |
750-810 |
APC-cy7, APC-H7 |
|
|
7 |
Violet (405nm) |
450/50 |
425-475 |
Pacific blue, Dapi, V450, BV421 |
|
8 |
502LP, 510/50 |
502-535 |
AmCyan, V500, BV510 |
How to read longpass filter detection range
How to read bandpass filter detection range
This instrument is capable of detecting up to 18 fluorescent parameters, making it ideal for multicolour flow cytometry assays that involve a large panel of markers. It comes with high throughput sampler unit for sample acquisition in multi-well plate.
Room 536, Basic Medical Science Building
University of Manitoba (Bannatyne campus)
FACSDiva
| Laser | Mirror/filter | Detection range | Flueorchrome | |
|---|---|---|---|---|
| 1 | Blue (488nm) | 505LP, 530/30 | 515-545 | FITC, GFP, Alexa488 |
| 2 | 685LP, 710/50 | 685-735 | PerCP-Cy5.5 | |
| 3 | Red (640nm) | 670/14 | 663-667 | APC, Alexa647, Cy5 |
| 4 | 690LP, 730/45 | 708-752 | Alexa700 | |
| 5 | 750LP, 780/60 | 750-810 | APC-cy7, APC-H7 | |
| 6 | Violet (405nm) | 450/50 | 425-475 | Pacific blue, Dapi, V450, BV421 |
| 7 | 505LP, 525/50 | 505-550 | AmCyan, CFP, V500, BV510 | |
| 8 | 600LP, 610/20 | 600-620 | BrilliantViolet605 | |
| 9 | 630LP, 670/30 | 655-685 | BrilliantViolet650 | |
| 10 | 690LP, 710/50 | 685-735 | BrilliantViolet711 | |
| 11 | 750LP, 780/60 | 750-810 | BrilliantViolet786 | |
| 12 | Yellow-Green laser (561nm) | 545LP, 586/16 | 578-594 | PE |
| 13 | 600LP, 610/20 | 600-620 | PE-Taxas Red | |
| 14 | 635LP, 670/30 | 655-685 | PE-Cy5 | |
| 15 | 750LP, 780/60 | 750-810 | PE-Cy7 | |
| 16 | UV laser (355nm) | 379/28 | 365-393 | BUV395 |
| 17 | 450LP, 515/30 | 500-530 | BUV496 | |
| 18 | 690LP, 740/35 | 723-757 | BUV737 |
How to read longpass filter detection range
How to read bandpass filter detection range
This Spinning Disc Confocal Microscope can accommodate for both tissue or cell imaging on glass slides or live cell imaging with chamber slides or AutoFluor cell chamber.
Room 466, Apotex Centre
University of Manitoba (Bannatyne campus)
It is equipped with stage heating system and connected to CO2, which ensures the cells will remain in optimal condition during the live cell imaging.
It is also capable of performing z-stacking, Tiles, FRAP and TIRF.
Its dual camera system allows for simultaneous image acquisition of GFP and RFP.
10x (air), 20x (air), 40x (water immersion), 63x (oil immersion)
| Name | Position | Bandpass | Emission range (nm) | Compatible flurochrome |
|---|---|---|---|---|
| Alexa488 | 1 | BP 525/50 | 500-550 | GFP, FITC, Alexa488 |
| Alexa488 + Alexa647 | 2 | DBP 527/54+647/60 | 500-554, 615-675 | Alexa488, Alexa595, Alexa647 |
| DAPI | 3 | BP 460/50 | 435-485 | DAPI |
| GFP | 4 | BP 525/50 | 500-550 | GFP, FITC, Alexa488 |
| Quad 446, 523, 600, 677 | 5 | 423-465, 500-550, 580-625, 660-695 | DAPI,Alexa488, Alexa595, Alexa647 | |
| TagRFP | 6 | 570-640 |
| Position | Bandpass | Emission range (nm) | Compatible fluorochrome | |
|---|---|---|---|---|
| 1 | 592-667 |
Flow Cytometry
413 Apotex Center, 750 McDermot Avenue
University of Manitoba
Winnipeg, MB, R3E 0T5, Canada
