Nediljko (Ned) Budisa

Research and teaching interests

    • Adaptive laboratory evolution for synthetic cells
    • Biocatalysis, biochemistry and biotechnology
    • Bioorthogonal chemistry and orthogonalization of life processes
    • Biophysics and biophysical chemistry
    • Classical enzyme engineering, protein and proteome engineering
    •  Classical peptide and protein physical organic chemistry
    • Classical organic and peptide syntheses
    • Directed evolution of synthetic enzymes and cells (with classical and machine learning-assisted gene library design)
    • Genetic code reprogramming and expansion
    • Genome editing and gene integration
    • Molecular microbiology
    • Metabolic engineering (synthetic metabolism, orthogonalization of metabolism)
    • Non-Canonical Amino Acids (ncAAs, chemistry, biochemistry and physiology)
    • Orthogonal enzymes and orthogonalization of protein translation
    • Protein stability, folding and design
    • Synthetic biology and xenobiology
    • Social, cultural, educational, ethical and philosophical aspects of synthetic biology and xenobiology (orthogonal life)

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