One of the most common uses of optical imaging is preclinical studies of anti-tumour agents. In the example shown here, fluorescence imaging was used to study the binding of a novel antitumour antibody, NovoMab-G2-scFv, to tumour cells. NovoMab-G2-scFv was labelled with Cy5.5.18, a fluorescent dye with an absorption maximum at 670 nm and emission maximum at 720 nm.


Perkin Elmer/Caliper IVIS Spectrum

Optical imaging is performed using a Perkin Elmer/Caliper IVIS Spectrum optical imaging system.

Additional information

Fluorescence and bioluminescence images are acquired using a high sensitivity, back thinned, back illuminated CCD camera cooled to -90 degrees Celsius.

Spectral region selected is enabled by 10 narrow band excitation (430, 465, 500, 535, 570, 606, 640, 675, 710 and 745 nm, 30 nm bandpass) and 18 narrow band emission (500, 520, 540, 560, 580, 600, 620, 640, 660, 680, 700, 720, 740, 760, 780, 800, 820 and 840 nm, 20 nm bandpass) filters, optimized to allow data acquisition from the blue to near infrared wavelength regions (430 nm to 850 nm).

For fluorescence imaging, the IVIS Spectrum allows investigators to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate experimental animals. Using a combination of a structured light source and trans-illumination investigators can perform 3D diffuse fluorescence tomography to determine source localization and concentration.

Four fields of view, (22.5 cm, 12.5 cm, 6.5 cm, and 3.9 cm) are selectable, allowing studies of up to five mice simultaneously or a single sample with high spatial resolution (up to 20 um).


Applications include: 

  • Non-invasive imaging of animal models of human disease
  • Non-invasive imaging of genetically engineered animals
  • Assess efficacy, pharmacokinetics and biodistribution of novel pharmacological agents
  • Assess novel drug delivery and gene therapy approaches
  • Develop new optical reporters for diagnostic imaging


Application notes

The materials listed here are available for investigators by request. 

Adaptive fluorescence background subtraction


Background ROI

Determine saturation

DLIT1 setup

DLIT2 topography

DLIT3 reconstruction

Drawing ROIs

FLIT 1 setup

FLIT 2 topography

FLIT 3 reconstruction

FMT imaging techniques

FMT multispecies imaging module (MSIM) for FMT

High resolution images

Image math

Image overlay 2D

Image overlay 3D

Imaging wizard

Load as group

Sending large files for analysis

Spectral unmixing

Subject ROI

Transillumination 1 setup

Transillumination 2 raster scan

Transillumination 3 normalized

Well plate quantification

Bibliographies by discipline

The materials listed here are available for investigators by request. 

Angiogenesis bibliography

Cardiovascular bibliography

Diabetes bibliography

Fluorescence imaging bibliography

Gene therapy bibliography

ID bibliography

Immunology bibliography

Inflammation bibliography

Nature and science publications

Neuroscience bibliography

Oncology bibliography

Rat models bibliography

RNAi bibliography

Stem cell bibliography

Transplantation studies and immunology bibliography

Virology bibliography

Xenogen all references bibliography


The materials listed here are available for investigators by request. 

IVIS Spectrum manual

PowerPoint Presentations

The materials listed here are available for investigators by request. 

IVIS advanced training presentation by Dr Brent Coco, Perkin Elmner Corporation

Selected papers

The materials listed here are available for investigators by request.

An intramolecular folding sensor for imaging estrogen receptor–ligand interactions

Building and breeding molecules to spy on cells and tumours

Combinatorial library screening for developing an improved split-firefly luciferase fragment-assisted complementation system for studying protein-protein interactions

Detection of protein-protein interactions in live cells and animals with split firefly luciferase protein fragment complementation

Determination of the background emission in mice

Development of a dual-luciferase reporter system for in vivo visualization of microrna biogenesis and posttranscriptional regulation

Fetal gene transfer using lentiviral vectors: In vivo detection of gene expression by micropet and optical imaging in fetal and infant monkeys

In vivo bioluminescence imaging of transplanted islets and early detection of graft rejection

In vivo imaging using bioluminescence: a tool for probing graft-versus-host disease

Molecular imaging of homodimeric protein–protein interactions in living subjects

Molecular imaging of the efficacy of heat shock protein 90 inhibitors in living subjects

Monocytes give rise to mucosal, but not splenic, conventional dendritic cells

Noninvasive optical tracking of red fluorescent protein–expressing cancer cells in a model of metastatic breast cancer

Quantitative fluorescence tomography validated with automated registration to 3D volumetric CT data

Real-time imaging of astrocyte response to quantum dots: In vivo screening model system for biocompatibility of nanoparticles

Revealing lymphoma growth and the efficacy of immune cell therapies using in vivo bioluminescence imaging

Three-dimensional reconstruction of in vivo bioluminescent sources based on multispectral imaging

Time course of bioluminescent signal in orthotopic and heterotopic brain tumours in nude mice

Tumour irradiation increases the recruitment of circulating mesenchymal stem cells into the tumour microenvironment

Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing

Contact us

Small Animal and Material Imaging Core Facility (SAMICF)
23 Basic Medical Sciences Building
745 Bannatyne Avenue
University of Manitoba
Winnipeg, MB R3E 0J9 Canada