· antimicrobial resistant bacterial and fungal pathogens
· mechanisms of antimicrobial resistance
· genomic epidemiology
· genomics-based innovation including WGS from clinical specimens and prediction of resistance from WGS
I am studying mechanisms of antimicrobial resistance and genomic epidemiology of various bacterial and fungal pathogens, including Enterobacteriales and Candida. I am interested in prediction of antimicrobial resistance from genome sequences and emerging resistance in Salmonella, especially resistance to extended spectrum beta-lactamases and gentamicin. I am also exploring methods to carry out whole genome sequencing directly from patient specimens.
There are an estimated 3000 invasive fungal infections each year in Canada, and two-thirds of these are caused by Candida species (5.8 cases per 100 000 population). There has been a global campaign to strengthen research and surveillance of fungal pathogens, which was prompted in part by the emergence of Candida spp with resistance to our limited arsenal of antifungal drugs.
We are carrying out a genomic study of Candida spp in Canada to understand the genomic epidemiology and mechanisms of antifungal resistance. This will be important for guiding treatment and antifungal stewardship. Whole genome sequencing can also aid infection control by detecting clusters of infections with a potential common source of contamination.
SELECT RECENT PUBLICATIONS
For a list of publications available on PubMed, click here
1. Walkty A, Karlowsky JA, Baxter MR, Adam HJ, Boyd D, Bharat A, Mulvey MR, Charles M, Bergevin M, Zhanel GG. 2018. Frequency of 16S ribosomal RNA methyltransferase detection among Escherichia coli and Klebsiella pneumoniae clinical isolates obtained from patients in Canadian hospitals (CANWARD, 2013-2017). Diagn Microbiol Infect Dis.
2. Mulvey MR, Bharat A, Boyd DA, Irwin RJ, Wylie J. 2018. Characterization of a colistin-resistant Salmonella enterica 4,,12:i:- harbouring mcr-3.2 on a variant IncHI-2 plasmid identified in Canada. J Med Microbiol 67:1673–1675.
3. Walkty A, Adam H, Tran V, Alexander DC, Boyd D, Bharat A, Karlowsky J. 2018. Failure of a multiplex polymerase chain reaction assay to detect IMP-27 in a clinical isolate of Morganella morganii. Diagn Microbiol Infect Dis 92:194–195.
4. Bharat A, Martin I, Demczuk W, Allen V, Haldane D, Hoang L, Mulvey MR. 2016. Complete Genome Sequences of Neisseria gonorrhoeae with Coresistance to First-Line Antimicrobials. Genome Announc 4:e00966–16.
5. Demczuk W, Martin I, Peterson S, Bharat A, Van Domselaar G, Graham M, Lefebvre B, Allen V, Hoang L, Tyrrell G, Horsman G, Wylie J, Haldane D, Archibald C, Wong T, Unemo M, Mulvey MR. 2016. Genomic Epidemiology and Molecular Resistance Mechanisms of Azithromycin-Resistant Neisseria gonorrhoeae in Canada from 1997 to 2014. J Clin Microbiol 54:1304–1313.
6. French S, Mangat C, Bharat A, Côté J-P, Mori H, Brown ED. 2016. A robust platform for chemical genomics in bacterial systems. Mol Biol Cell 27:1015–1025.
7. Bharat A, Martin I, Zhanel GG, Mulvey MR. 2016. In vitro potency and combination testing of antimicrobial agents against Neisseria gonorrhoeae. J Infect Chemother 22:194–197.
8. Peterson SW, Martin I, Demczuk W, Bharat A, Hoang L, Wylie J, Allen V, Lefebvre B, Tyrrell G, Horsman G, Haldane D, Garceau R, Wong T, Mulvey MR. 2015. Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens. J Clin Microbiol 53:3606–3608.
9. Peterson SW, Martin I, Demczuk W, Bharat A, Hoang L, Wylie J, Allen V, Lefebvre B, Tyrrell G, Horsman G, Haldane D, Garceau R, Wong T, Mulvey MR. 2015. Molecular Assay for Detection of Genetic Markers Associated with Decreased Susceptibility to Cephalosporins in Neisseria gonorrhoeae. J Clin Microbiol 53:2042–2048.
10. Bharat A, Demczuk W, Martin I, Mulvey MR. 2015. The effect of variants of penicillin-binding protein 2 (PBP2) on cephalosporin and carbapenem susceptibilities in Neisseria gonorrhoeae. Antimicrob Agents Chemother 59:5003–5006.