| MBIO
4600 Teaching Assistants:
Ben Bailey-Eilkin, Justin Hawkins, Amanpreet Singh
All lab reports must have a completed
Honesty Declaration attached (pdf): Group
Lab Assignment or Individual
Lab Assignment.
Lab starts Tuesday September 13, 2011 -in rooms 201 and 204 Buller. Lab manuals are handed out in class
the first week of lectures. All essential lab related information is given
in the lab manual.
Lab Exam Outline
Lab report marks
Lab exam mark and final lab marks
Report data sheets (Word):
Lab 4, Lab
5. Save and open in Word before entering requested information. OR
save, open, print and just enter data requested by hand.
Report lab format (Word): Lab 1, Lab
2, Lab 3, Lab
4. Save lab format and open in Word to enter requested information.
Report spreadsheets (Excel):
Lab 4 (class data is now available) and Lab 5 (class data now available. If required, spreadsheet contains supplied group transposition data worksheet). Save spreadsheet and open in Excel to enter
requested information. There may be more than one worksheet per file,
check tabs at bottom of worksheet to open additional worksheet. If required, group transposition data has been supplied - see additional tab. If using supplied group data, cite supplied data. Group data must be handed in even if zero colonies.
Report Data
2011 Lab 2 Papillae results for E coli CSH101 x E. coli CSH114 cross on lactose MacConkey or M9glucose Xgal IPTG (jpg files): Group Numbers: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28.
Lab 3 Agarose Gel Results (Hind111 digested plasmid):When inserting the jpg file into Word report format keep the entire picture - all groups - do not cut/paste - need ladder and other lanes and if necessary, resize to fit ~1/2 page.
If your group does not have a single band (expected) for pMR106 digest at the expected size, then most likely your DNA is undigested or partially digested - use another group's DNA to determine concentration (reference the group's data used). If no DNA in pMR106 lane, use another group's DNA to determine concentration (reference the group's data used). Due to culture problems with E. coli DH5α(pBluescript), no pBluescript plasmid isolated. pBluescript plasmid will be supplied next week.
2011 Tuesday Student Sign-up Sheets (pdf): 1, 2, 3, 4, 5, 6, 7, 8 Ignore lanes where pBluescript loaded.
2011 Tuesday Student Gel Photographs (jpg): gel 1 (group 10 reversed loading, lane 6 is really pMR106), gel 2, gel 3, gel 4, gel 5, gel 6, gel 7, gel 8 (group 28 reversed loading, lane 4 is really pMR106) Ignore lanes where pBluescript loaded.
Information and References
Lab 2
Papillae examples:
X-gal plate - blue papillae (dots) arise in the Lac- colony. Papillae arise from a single cell in the colony that has reverted to Lac+. Data is from group 33, 2010 lab.
MacConkey Plate - red papillae (dots) arise in the Lac- colony. Data is from group 36, 2010.
Cupples, C.G. & Miller, J.H. 1989. A set of lacZ mutations in Escherichia coli that allow rapid detection of each of the six base substitutions. PNAS 86:5345-5349.
General
Berlyn, MKB. 1998. Linkage
map of
Escherichia coli K-12, Edition 10: The Traditional Map. Micro Mol.
Biol. Rev. 62: 814-984. or search ASM journals or UM Google Scholar
Faculty
of Science Cheating, Plagerism,etc
Frequently information is available as
a pdf file requiring Adobe Acrobat Reader. Adobe abcrobat reader is available
at UM
Software Express.
Lab Skill Sheet-
available for students to use for job applications, just check off the
highlighted boxes for the lab course(s) you have taken.
pdf or
excel
email le_cameron@umanitoba.ca
Emails: subject must contain course number and subject,
eg. 4600 lab 1 report. If no subject given, email is deleted. Emails replies
occur only during working hours. Email must include student name.
phone 474-6542
Department of Microbiology
Buller Bldg
Winnipeg, MB
R3T 2N2
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