MBIO 4520
Industrial Bioprocesses Lab

MBIO 4510 Teaching assistants:
Jennifer Tanner and Chris Maltman

All lab reports must have a completed Honesty Declaration attached: Group Lab Assignment or Individual Lab Assignment. Available as pdf.

Lab starts Friday, January 13, 2012 in rooms 201 and 204 Buller. Lab manuals are handed out in class the first week of lectures. All essential lab related information is given in the lab manual.

Read lab report presentation section in general instructions at the beginning of your lab manual before doing your lab report. Lab reports must be typed. This includes calculations. 10% of the mark subtracted for portion of report not typed.

Lab Report Word Documents: Lab 1, Lab 2 (Initial Experiment Design sheet)

Lab Report Excel spreadsheets: Lab 1, Lab 2, Lab 3.

Lab References:
Majority of articles available on-line, listed below. Articles not available on-line (textbooks excerpts - see reference table in lab manual) are available in MBIO 4510 lab reference binder on 2h reserve in Science and Technology Library. If a reference required for a question is not linked below, just use UM Goggle Scholar.


Lab 2 (selected references)
Haq, I, Ali, S, Qadeer, MA, Iqbal, J. 2003. Stimulatory effect of alcohols (methanol and ethanol) on citric acid productivity by a 2-deoxy D-glucose resistant culture of Aspergillus niger GCB-47. Bioresource Technology 86: 227-233.

Halliwell, G and TK Walker. 1951. The Metabolism of Glucose and Acetate in Aspergillus niger. J. Exp. Botany 3:155-161.

Hesse, SJA, Ruijter, GJG, Dijkema, C, Visser, J. 2002. Intracellular pH homeostatis in the filamentous fungus Aspergillus niger. Eur. J. Biochem. 269: 3485-3494.

Karaffa, L, Kubicek, CP. 2003. Aspergillus niger citric acid accumulation: do we understand this well working black box? Appl. Microbiol. Biotechnol. 61: 189-196.

Kubicek, CP and M. Rohr 1977. Influence of Manganese on Enzyme Synthesis
and Citric Acid Accumulation in Aspergillus niger. Appl. Microbiol. Biotechnol.4: 167-175. Journal previously called European J. Appl. Microbiol.

Kubicek,C.P. Zehentgrubcr, O.E1-Kalak, Housam and M. Rohr 1980. Regulation of Citric Acid Production by Oxygen:Effect of Dissolved Oxygen Tension on Adenylate Levels and Respiration in Aspergillus niger. Applied Microbiology and Technologyl. 9: 101-115.

Legisa, M., Gradisnik-Grapulin, M. 1995. Sudden substrate dilution induces a higher rate of citric acid production by Aspergillus niger. Appl. Env. Micro. 61: 2732-2737.

Roukas, T. 2000. Citric and gluconic acid production from fig by Aspergillus niger using solid-state fermentation. J. Ind. Micro. & Biotech. 25:298-304.

Ruijter, GJG., van de Vondervoort, PJI., Visser, J. 1999. Oxalic acid production by Aspergillus niger: an oxalate-non-producing mutant produces citric acid at pH 5 and in the presence of manganese. Microbiology. 145: 2569-2576.

Xu, D-B, Madrid, CP, Rohr, M, Kubicek, CP. 1989. The influence of type and concentration of the carbon source on production of citric acid by Aspergillus niger. Appl Microbiol Biotechnol 30: 553-558

Xu, D-B, Kubicek, CP 1, and M Riih. 1989. A comparison of factors influencing citric acid production by Asperyillus niger grown in submerged culture and on filter paper. Appl Microbiol Biotechnol. 30:444-449

Yigitoglu, M., McNeil, B. 1992. Ammonium ion & citric acid supplementation in batch cultures of Aspergillus niger. Biotechnology Letters. 14:831-836.

Tissue Culture Tour
Location: Hallway outside 126 Buller
Group tour times: groups 1 to 5 at 2:30 pm, groups 6 to 10 at 2:45 pm and groups 11 to 14 at 3:00 pm.
Date: TBA
Cedex information (not responsible for comparison information)

Fort Garry Brewery Tour
Location: 130 Lowson Cr (route map)- map is also in your lab manual
Date:TBA
Time: 2:00 pm (be there at least 5 to 10 min early) or as soon as possible for students that are attending class before lab. Usually there are two tours.
Transportation: you are responsible for transportation, although I have 3 spaces available in my car (please discuss with me ahead of time)
Fort Garry Brewing Co. Ltd.
Attendance is not compulsory. If attending, you MUST complete a Release form (appendix of lab manual) and hand into report slot (hall opposite 302 Buller). To print a separate sheet of release form - use pdf lab manual available on lab website to print page.

Frequently information is available as a pdf file requiring Adobe Acrobat Reader. Adobe abcrobat reader is available at UM Software ExpressUM Software Express.

Faculty of Science Cheating, Plagerism,etc

Lab Skill Sheet- available for students to use for job applications, just check off the highlighted boxes for the lab course(s) you have taken.
pdf or excel (prior to Fall 2007)
pdf or excel (starting Fall 2007)

email le_cameron@umanitoba.ca
Emails: subject must contain course number and subject, eg. 4510 lab 1 report. If no subject given, email is deleted. Emails replies occur only during working hours. Email must include student name.

phone 474-6542
Department of Microbiology
Buller Bldg
Winnipeg, MB
R3T 2N2