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MBIO 3480 Teaching
assistants:
Aniel Moya Torres, Thomas Reeves, Chris Maltman, Jennifer Tanner
All lab reports must have a completed
Honesty Declaration attached: Group
Lab Assignment or Individual
Lab Assignment. Available as pdf.
Lab starts Thursday September 15, 2011 in room 302 Buller. Lab manuals are handed out in class
the first week of lectures. All essential lab related information is given
in the lab manual.
Lab Exam Outline
Lab report marks
Lab exam mark and final lab marks
LAB INFORMATION
Class is divided alphabetically by last name into
TWO sections:
Section 1 (A-L) starts at 2:30 pm
Section 2 (M-Z) starts at 4:00 pm (some weeks may start earlier but not
week - check the schedule for exceptions)
If you want to switch sections, find someone in the other section to switch
with, then see me in room 414B Buller. There are only 30 seats and both
sections are full - last names checked the first day. May still need to
balance sections the first day of lab.
All basic information you need to know is in your lab manual.
I expect you to check the website on a regular bases.
Before coming to the first lab:
Prepare for practical phase contrast microscope quiz by reading microscope operation in appendix. Details given in lab manual - see content list.
Read list of possible projects - pick one from your section's list as first come, first served.
Read general instructions including lab report presentation (reports must be typed).
Read week 1 lab procedures.
Lab Data and Report
formats (Word): Soil Microbial Community
Project Experiment Outline, Soil Microbial Community Project Procedure Report, Lab
1 report, Lab 2
Ecoplate data sheet, Lab 4 Report. Save
Word format and open in Word to enter requested information. Concentration of Erythromicrobium ramosum E5 culture: 3.2 mg/ml.
Clarification: Lab 4 report due Thursday November 10.
Report spreadsheets (Excel): Lab
2 (class data is now available). Save spreadsheet and open in Excel to enter requested information. Caution when adding standard deviation bars to class data figure, the y-axis may automatically reset to minus value - change back to zero by right clicking on primary vertical axis.
Lab 3 Anoxygenic Phototrophic Bacteria: Photosynthetic Pigments Student data
TLC plates (acetone extracted samples). After opening copy/paste
jpg file into a Word document and if too large resize to fit. Your data may not be as expected - check other group's data. If your data is poor use another group's data and reference. Remember you want bacteriochlorophyll not just carotenoids.
group 1, group 2, group 3, group 4, group 5, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24, group 25, group 26.
Biochrom Excel spreadsheets
Live Rhodospirillum rubrum absorbance scans (320 - 900 nm):group 1, group 2, group 3, group 4, group 5, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24, group 25, group 26.
Acetone extracted Rhodospirillum rubrum and Winogradsky absorbance scans (320 - 900 nm): group 1, group 2, group 3, group 4, group 5, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24, group 25, group 26.
Lab 4 Aerobic Phototrophs Student Data
Erythromicrobium ramosum E5 protein concentration of original culture: 3.2 mg/ml (2011)
TLC plates (acetone extracted samples). After opening copy/paste
jpg file into your lab report Word document and if too large resize to fit.
group 1, group 2, group 3, group 4, group 5, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24, group 25, group 26.
Biochrom Excel spreadsheets
Live Erythromicrobium ramosum E5 live scan (320-600 nm): group 1, group 2, group 3, group 4, group 5, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24, group 25, group 26. If your group does not have the expected peaks, just note the expected peaks for live E. ramosum E5 in the title.
Acetone extract of the two dominant carotenoids in Erythromicrobium ramosum (320 - 600 nm):
group 1, group 2, group 3, group 4, group 5, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24, group 25, group 26.
Soil Diversity Project Pictures (jpg)
Lab
References and Additional Information
Microbial Soil Diversity Project
Environmental Microbiology
Lab Manual (2003) - possible source of information.
Lab 1 Nitrogen fixation
Picture
of Azotobacter cyst Wyss, O, Neumann, M, Socolopsky, M. 1961. Development
and germination of the Azotobacter Cyst. J. Biophysical and Biochemical
Cytology. 10: 555-565.
Bean plant root nodules: There are two types of nodules,
determinate which are round and indeterminant which branch from a central
corel line. See picture below for closeup of the different types of nodules
on a selection of legumes. Plants left to right, clover, two large broad
bean plants (pea family), bush bean, and pea plant.
Lab 2 Functional Microbial Diversity
Biolog
Microbial Community Analysis
ANOVA analysis for Independent Samples via VassarStats: Statistical Computation Website homepage.
See lab 2 appendix for additional instructions. If you encounter problems, use a different
browser or change settings. As
stated in your lab manual you need to remove any empty spaces at end of
data. This also applies to empty spaces in middle of data column (eg group
data information not submitted). Again just backspace to remove any empty
lines before importing data.
Lab 4 Aerobic anoxygenic phototrophic
bacteria
Concentration of Erythromicrobium ramosum E5 culture: 3.2 mg/ml
Yurkov,
V, Beatty, T 1998. Aerobic anoxygenic phototrophic bacteria. Micro.
Mol. Biol. Rev. 62: 695-724. [ASM journal]
PROJECT PRESENTATION
INFORMATION - Thursday Nov 17, 2011
Please bring a USB stick containing your
Powerpoint presentation. Computer provided. Refer to schedule for presentaton time information.
Schedule
2011
Marking outline
Phase Contrast Microscope
Eclipse E100
phase contrast microscope with condenser
close up
Eclipse
E100 Nikon link
Faculty
of Science Cheating, Plagerism,etc
Frequently information
is available as a pdf file requiring Adobe Acrobat Reader. Adobe abcrobat
reader is available at UM Software ExpressUM
Software Express.
Lab Skill Sheet-
available for students to use for job applications, just check off the
highlighted boxes for the lab course(s) you have taken.
pdf or
excel (starting Fall 2007)
email le_cameron@umanitoba.ca
Emails: subject must contain course number and subject,
eg. 3480 lab 1 report. If no subject given, email is deleted. Emails replies
occur only during working hours. Email must include student name.
phone 474-6542
Department of Microbiology
Buller Bldg
Winnipeg, MB
R3T 2N2
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