| MBIO
3470 Teaching Assistants:
Thomas Reeves- 401 Buller (scheduled lab time)
Mohamed Abdel-Fattah, Elizabeth Hughes, Aneil Moya Torres (during the week
supervisors)
All lab reports must have a completed
Honesty Declaration attached: Group
Lab Assignment or Individual
Lab Assignment. Available as pdf.
Lab 1 Mixed Culture marks
MBIO 3470 LAB INFORMATION
Lab starts Wednesday, January 11, 2012 in rooms
302 and 304 Buller at 2:30 pm for all students. Lab manuals are handed out in class the first week of lectures.
All essential lab related information is given in the lab manual.
The first lab starts with a Standard Operations Procedure (SOP) and BIOSAFETY QUIZ (15 min) - must pass to continue lab (Refer to lab 1 outline in lab manual for list of
information covered in quiz.)
Come prepared the first lab ready to comply with all standard
operations procedures.
Reminders:
You need a separate lab coat for MBIO 3470 that stays in the lab. You
must bring your lab coat the first lab and a bag to hold your lab coat.
No outer clothing or back packs allowed in the lab, only lab manual, notebook
and pens. Small lockers available in the hall outside the lab. Long hair
must be tied back. No open toed shoes.
Lab Safety:
UM
Environmental Health and Safety Office (EHSO) -
chemical and biological safety
Basic
Lab Safety and WHMIS (PowerPoint presentation)
Biohazard
MSDS
Biological Safety Cabinet Manual - Labconco Purifier CELL Logic
Read lab report presentation section in general instructions at the beginning of your lab manual before doing your lab report. Lab reports must be typed. This includes calculations. 10% of the mark subtracted for portion of report not typed.
Data Collections (Word format): lab 4 isolation data sheet. Save
Word document and open in Word before recording requested information.
LAB REPORT (Word format):
lab 1 Table 1. Biochemical identification of an unknown Enterobacteriaceae, lab
2, lab 3, lab
4, lab 5 and lab 6. Save
Word document and open in Word before recording requested information.
LAB REPORT (Excel format):
lab 1 Numerical data
sheet. Save spreadsheet and open in Excel before recording requested
information. The spread sheet has been set to automatically give you the
total numerical value for each bacterium.
Microbial Systematics Lab Manual
(numerous tables/diagrams found in lab manual).
Lab 1 Table 1. Biochemical
Characteristics of selected members of Enterobacteriaceae (pdf)
Lab 1 Table 2. Biochemical
Characteristics Data Sheet (pdf)
Blank API 20E sheet (data collection only)
Lab 3 API Identification Table: page
1 and page 2
Lab 6 blank API 20NE
data sheet (data collection only)
Gram Stain addendum - the company has notified us that the safranin counter
stain contains gram positive coccoid artifacts. The coccoids are artifacts
of the stain, not culture contamination.
LAB 5 Streptococcaceae
Spanu,
T, Sanguinetti, M, Ciccaglione, D, D’Inzeo, T, Romano, L, Leone,
F, Fadda, G. 2003. Use of the VITEK 2 System for rapid identification
of clinical isolates of Staphylococci and bloodstream infections. J. Clin.
Micro. 41: 4259-4263
Wellinghausen,
N, Wirths, B, Essig, A, Wassill, L. 2004. Evaluation of the Hyplex
Bloodstream Multiplex PCR-enzyme-linked immunosorbent assay system for
direct identification of Gram-postive cocci and Gram-negative bacilli
from positive blood cultures. J. Clin. Micro. 42: 3147-3152.
Biosafety
MSDS (infectious
agents)
Faculty
of Science Cheating, Plagerism,etc
Frequently information is available as
a pdf file requiring Adobe Acrobat Reader. Adobe abcrobat reader is available
at UM Software ExpressUM
Software Express.
Lab Skill Sheet-
available for students to use for job applications, just check off the
highlighted boxes for the lab course(s) you have taken.
pdf or excel (prior to Fall 2007)
pdf or
excel (starting Fall 2007)
email le_cameron@umanitoba.ca
Emails: subject must contain course number and subject,
eg. 3470 lab 1 report. If no subject given, email is deleted. Emails replies
occur only during working hours. Email must include student name.
phone 474-6542
Department of Microbiology
Buller Bldg
Winnipeg, MB
R3T 2N2
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