MBIO 3460
Membrane and Cellular Biochemistry Lab

MBIO 3460 Teaching assistants:
Tuhin Guha and Anna Motnenko

All lab reports must have a completed Honesty Declaration attached: Group Lab Assignment or Individual Lab Assignment. Available as pdf.

Lab starts Friday September 16 (Section 1) and Sept 23 (Section 2), 2011 in rooms 201 and 204 Buller. See lab information below and lab manual schedule to determine your start time. Lab manuals are handed out in class the first week of lectures. All essential lab related information is given in the lab manual.

Lab Exam Outline

Lab report marks

Lab exam mark and final lab marks

LAB INFORMATION
Class is divided alphabetically by last name into TWO sections:
Section 1 (M-Z) - see lab manual schedule for lab times.
Section 2 (A-L) - see lab manual schedule for lab times.
If you want to switch sections, find someone in the other section to switch with, then see Dr. Cameron in room 414B Buller.
All basic information you need to start the lab is found in the lab manual. Also check the lab website on a regular bases.

Lab Reference Material
Lab 1:TLC.
Lab 2: Erythrocyte Membrane Proteins: Barker, RN 1991 Electrophoretic analysis of erthrocyte membrane proteins and glycoproteins from different species. Comparative Haematology International 1:155-160. (pdf file)
Acrylamide polymerization - a practical approach by Paul Menter, Bio-Rad Laboratories

Read lab report presentation section in general instructions at the beginning of your lab manual before doing your lab report. Lab reports must typed. This includes calculations. 10% of the mark subtracted for portion of report not typed.

Lab 1 The isolation and quantitation of ubiquinone Report:

Lab 1 Report format (Word). Save file and open in Word before entering required information.

Group Biochrom ubiquinone scan absorbance data (Excel): group 1, group 2, group 3, group 5, group 6, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24. If reduced scan is not less than the oxidized scan at 275 nm then you need to borrow another group's data and reference for lab report. Also if you have negative oxidized scan at 275 nm, then you need to borrow another group's data and reference for lab report.

Lab 1 TLC results: group 1, group 2, group 3, group 5, group 6, group 7, group 8, group 9, group 10, group 11, group 12, group 13, group 14, group 15, group 16, group 17, group 18, group 19, group 20, group 21, group 22, group 23, group 24.
After opening copy/paste jpg file into your lab report Word document and if too large resize to fit.

Lab 2 Red Blood Cell Membrane Proteins:

Lab 2 Report format (Word). Save file and open in Word before entering required information.
Lab 2 Report Excel spreadsheet (protein concentration determination and protein molecular weight determination worksheet - tab at bottom of each worksheet). Save file and open in Excel before entering requested information.
SDS-PAGE group results: Insert jpeg files into word document - right click the picture, copy image and paste in Word document.If your group's data is too light or too dark use another group's data but remember to state borrowed data and give group #.
SDS-PAGE gel loading plan(pdf): Lab 204 Section 1, gel 1 and gel 2 (see gel lane label for final order) and Lab 201 Section 2, gel 1 and gel 2 (see gel lane label for final order). Note, gel 1 for lab 204 section 1 has part of the Benchmark protein ladder photo-shopped due to damage to part of ladder during drying.
SDS-PAGE gels: Section 1 (Room 204) gel 1 and gel 2, Section 2 (Room 201) gel 1 and gel 2. When correlating the bands on the BenchMark protein ladder (Ladder) with kDa size, use the intense bands (20 and 50 kDa) to match intense bands in lab manual figure of ladder. Room 204 (Section 1) ladder is missing the 15 and 10 kDa bands - have run off the bottom of both gel. All sections ignore light bands between 25 and 30 kDa and also between 40 and 50 kDa. On some gels there are other very light bands that should be ignored.

Some Possible Search Engines: ASM journal search, Goggle Scholar

Tutorial: EM Information

Lab Equipment:
Lab 1 reflux apparatus, homogenizer (close-up) and ether extraction

Faculty of Science Cheating, Plagerism,etc

Frequently information is available as a pdf file requiring Adobe Acrobat Reader. Adobe abcrobat reader is available at UM Software Express UM Software Express.

Lab Skill Sheet- available for students to use for job applications, just check off the highlighted boxes for the lab course(s) you have taken.
pdf or excel (prior to Fall 2007)
pdf or excel (starting Fall 2007)

email le_cameron@umanitoba.ca
Emails: subject must contain course number and subject, eg. 3460 lab 1 report. If no subject given, email is deleted. Emails replies occur only during working hours. Email must include student name.

phone 474-6542
Department of Microbiology
Buller Bldg
Winnipeg, MB
R3T 2N2

University of Manitoba calender 2008 -2009