MBIO 4440
Systems MIcrobiology:
from Genomes to Life Lab

MBIO 3440 Teaching assistants:
Ben Bailey-Elkin, Justin Hawkins & Anna Motnenko

All lab reports must have a completed Honesty Declaration attached: Group Lab Assignment or Individual Lab Assignment. Available as pdf.

Lab starts Tuesday, January 10, 2012 in rooms 201 and 204 Buller. Lab manuals are handed out in class the first week of lectures. All essential lab related information is given in the lab manual.

Read lab report presentation section in general instructions at the beginning of your lab manual before doing your lab report. Lab reports must be typed. This includes calculations. 10% of the mark subtracted for portion of report not typed.

Lab report format (Word): lab 1, lab 5 report questions. Save document, then open in Word to enter requestion information.

Lab report format (Excel): lab 2 (class data now available). Caution lab 2 standard curve, for the umole alpha-ketobutyrate column, make sure there are three decimal places to get the correct standard curve) this spreadsheet consists of 4 worksheets; degradataive standard curve, degradative, biosynthetic, class data , lab 3, lab 4, lab 5. Save first, then open in Excel to enter required information.
Excel spreadsheet may consist of numerous worksheets, see tabs at bottom of worksheet, use arrows to scroll between worksheets.

Data sheets (word format): lab 3, lab 4, lab 5. Save first, then open in Word to enter required information.

Student Report Information:
Lab 2 Plate Reader Data (Excel) for Degradative Threonine Deaminase crude extract colorimeter assay: all groups

Lab 2 Student Data:
Threonine deaminase crude extract protein concentrations (2012):
Degradative:_6.4___ mg/ml
Biosynthetic:__6.7___ mg/ml


Tutorial EM information

Lab Reference File
Use Goggle Scholar through UM eLibrairies, if link is not given below.

Lab 2
ECOCYC Encyclopedia of Escherichia coli K -12 Genes and Metabolism. Access information by put in the gene or protein in the search box.
Edward Eisenstein. 1991. Cloning, Expression, Purification, and Characterization of
Biosynthetic Threonine Deaminase from Escherichia coli. J. Biol. Chem. Vol. 266, No. 9, Issue of March 25. pp. 5801-5807.
YUTAKA SFIIZUTA, ZTSUSIII NAKAZAWA, MASANOBU TOIWSHIGE, AND OSAMU HAYAISHI 1969. Studies on the Interaction between Regulatory Enzymes and Effectors
III. CRYSTALLIZATION AND CHARACTERIZATION OF ADENOSINE 5’-MONOPHOSPHATE-DEPENDENT THREONNE DEAMINASE FROM EXCHERICHIA COLI J. Biol. Chem. Vol. 244,N o. 7, Issue of April 10, pp. 1883-18891.

Lab 3
Michel, G.P.F., & J. Starka. 1986. Effect of Ethanol and Heat Stresses on the Protein Pattern of Zymomonas mobilis Journal of Bacteriology. 165, 1040-1042. (ASM journal)
Neidhardt, F.C., VanBogelen, R.A. & V. Vaughn. 1984. The Genetics and Regulation of Heat-Shock Proteins. Ann. Rev. Genet. 18: 295-329 Available UM E-LIBRARY, Search the Web, Google scholar. Just type in title.
O 'Farrell, PH. 1975. High Resolution Two-Dimensional Electrophoresis of Proteins. J. Biol. Chem. 250: 4007-4021. Available UM E-LIBRARY, Search the Web, Google scholar. Just type in title.
VanBogelen, R. A., P. M. Kelley, and F. C. Neidhardt. 1987. Differential Induction of Heat Shock, SOS, and Oxidative Stress Regulons and Accumulation of Nucleotides in Escherichia coli. J. Bacteriology, 169:26-32. (ASM journal)

Lab 4
Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. Growth of Cells and Culture. Chapter 102. Timing of Synthetic Activities in the Cell Cycle by Charles E. Helmstetter. 2009 American Society for Microbiology.
Kubitschek, H. E. 1974 Estimation of the D Period from Residual Division after Exposure Phase Bacteria to Chloramphenicol. Molec. Gen. Genet. 135:123-130.
Donachie, W. D. 1969 Control of Cell Division in Escherichia coli: Experiments with Thymine Starvation. J. Bact. 100:260-268.

Lab 5
Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. Chapter 73. Chemotaxis by J.B. Stock and M.G. Surette. 2009 American Society for Microbiology.
Zhulin, IB, Rowsell, EH, Johnson, MS, Taylor, BL. 1997. Glycerol elicits energy taxis of Escherichia coli and Salmonella typimurium. J. Bact. 179: 3196-3201. (ASM journal)
Tso, Wung-Wai & J. Adler. 1974. Negative Chemotaxis in Escherichia coli. J. Bact. 118: 560-564, 566-569. (ASM journal)


Faculty of Science Cheating, Plagerism,etc

Frequently information is available as a pdf file requiring Adobe Acrobat Reader. Adobe abcrobat reader is available at UM Software ExpressUM Software Express.

Lab Skill Sheet- available for students to use for job applications, just check off the highlighted boxes for the lab course(s) you have taken.
pdf or excel (prior to Fall 2007)
pdf or excel (starting Fall 2007)

email le_cameron@umanitoba.ca
Emails: subject must contain course number and subject, eg. 3440 lab 1 report. If no subject given, email is deleted. Emails replies occur only during working hours. Email must include student name.

phone 474-6542
Department of Microbiology
Buller Bldg
Winnipeg, MB
R3T 2N2