IMMU7040 - Immunological Methodology
Course Outline
Winter 2018
CRN #52295   A01
Course Co-ordinator: Dr. Aaron Marshall
Address: 415 Apotex Centre
Phone: (204) 789-3385
Course Times: Wednesdays, 9:30-11:30 a.m.

NB:  Some lab components may run on the Wednesday afternoon of the lecture
Location: 477 Apotex Centre (A. Sehon Suite)


IMMU7040 - Immunological Methodology is a practical course covering a range of methodologies in common use in immunology and other biomedical research fields.

Lectures will cover (1) the theoretical basis of methods, (2) common applications in immunology, (3) practical pitfalls/common problems, (4) and the advantages and disadvantages of competing methodologies.

Exam format:  Take-home exam of experimental problems incorporating several of the covered methodologies.  Answers will be graded independently by at least 3 instructors and averaged.


Mid-term exam (take home) 30%
Final exam (take home) 70%


Date Topic Lecturer
January 10, 2018  Introduction to methodology/Leukocyte purification methods  A. Marshall
Jan. 17  Human immunology: integrating lab and population studies  K. HayGlass
Jan. 24  Enzyme immunoassays and their applications  R. Su
Jan. 26
 Flow cytometry
 Lab demo to follow 12:30-1:30 p.m., 4th Floor Apotex Centre
 C. Zhang
Feb. 7  RNA work, RT-PCR and quantitative analysis of gene expression
 Lab demo to follow lecture
 A. Soussi Gounni
Feb. 14  Gene microarrays: practical aspects and data analysis A. Raouf
Feb. 21  Proteomics / high content analysis and protein interactions
 Tour/demo at Manitoba Centre for Proteomics and Systems Biology - to be confirmed
N. Mookherjee
Feb. 26

Covers Lectures 1-7, worth 30% of your final mark.

Pick up your copy of the exam at 10:00 a.m. from
Karen Morrow, Administrative Assistant, Department of Immunology,
471 Apotex Centre

DUE DATE:  Wednesday, February 28, 2018 at 3:00 p.m..\ 
Return your exam to Karen Morrow.

March 7  Intravital microscopy
 Lab demo to follow lecture
 T. Murooka
March 14  Animal models for host-defense against pathogens  J. Uzonna
March 21  Animal models for inflammatory disease  J. Ghia
March 28  Methods for generation of antibodies binding a specific molecular target
 X. Han
April 4  Immunoprecipitation and electrophoretic analysis of proteins  S. Gibson
April 11  DNA manipulation / expression vectors / gene transfer methods
 Lab demo to follow lecture
 S. Kung
April 18  Immunohistology, immunofluorescence and confocal microscopy
 Lab demo to follow 12:30-1:30 pm
 A. Marshall
April 25  Functional analysis of genes in cells and organisms  A. Marshall
April 27  Pick up FINAL EXAM from Immunology administrative office (471 Apotex Centre) at 11:30 a.m.
 Due:  4:00 p.m., Friday, May 4