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Chimera Generation
CRISPR/Cas9 is now the method of choice for the generation of most mouse models. Our facility has successfully used CRISPR/Cas9 to generate single and double knock-in mice as well as knock-out mice. Pricing for CRISPR/Cas9 modifications differ depending on the complexity of the model to be produced, including the number of targeting events that are involved in constructing the model. Both microinjection and ES cell aggregation services are also available to produce chimeric mice but are no longer routinely used within the facility.