Neural stem cells are the basic building blocks of the nervous system. Once thought to be only of importance during early CNS development, it is now known that many neural stem cell populations persist lifelong in the vertebrate brain and spinal cord. In some cases these neural stem cells remain in a dormant state, whereas others can be found actively dividing in restricted compartments of the brain, commonly known as the neurogenic niche. The heterogeneous nature of neural stem and progenitor cells throughout the vertebrate CNS has questioned the role of these distinct cell populations, and the mechanism regulating their behaviour during development, adulthood, and following injury.
In the LINDSEY LAB we use modern cellular and molecular approaches to investigate how unique neural stem and progenitor populations behave in a context-dependent manner. To address this question we take advantage of the unique features of the zebrafish model to study neural stem and progenitor cells from early development to senescence, and during the reparative process following neuro-trauma.
1. Stem cell niche development and lifelong changes in the cellular and molecular features of neural stem and progenitor cells.
2. The regenerative potential and regulation of niche-specific neural stem cell populations.
3. Neural stem cell plasticity and function during animal behavior and learning.
To uncover these themes, we combine advanced imaging with multiple cellular, molecular and behavioural techniques. Trainees joining the lab will have the opportunity to gain expertise in procedures and techniques in the zebrafish model such as:
ANIMAL: husbandry, anesthetization, brain and spinal cord lesion assays, dissection, micro-injection
CELLULAR: proliferation, cell death, and senescence assays, lineage tracing, immunohistochemistry, tissue preparation, cryosectioning, tissue clearing
MOLECULAR: in situ hybridization, PCR, transgenesis, RNA-sequencing and analysis
IMAGING: brightfield, fluorescence, confocal microscopy, 3-D imaging techniques, electron microscopy, correlative fluorescence electron microscopy, live in vivo imaging, image analysis
BEHAVIOUR: behavioural design, sensory assays, advanced behavioural analysis.
JOIN OUR TEAM AND BE A PART OF THE NEXT DISCOVERY IN NEURAL STEM CELL BEHAVIOUR AND REGENERATIVE MEDICINE
We are always looking for highly motivated students and post-docs to join our team. The LINDSEY LAB offers high quality scientific training and academic mentorship in a supportive learning environment.
Interested candidates should directly email Dr. Benjamin Lindsey at Benjamin.email@example.com to learn about available projects within each research theme.
For more information on the LINDSEY LAB, visit the lab website at lindseylab.ca (currently under construction).