Lentiviral Vector Viral Particles Production Core Platform
Location: 4th Floor Apotex Centre
Sam Kung, Jiuyong Xie
Understanding how a protein functions in complex biological processes is key to basic biomedical research and identification of novel drug targets in human diseases. Replication-incompetent HIV-1 based lentiviral vectors have become widely popular in the manipulations and functional analyses of a protein of interest in cells. Key to the successful uses of lentiviral vectors in these applications is the ability to produce high quality and high-titered lentiviral particles. The latter demands skills and a well-maintained system that can be time-consuming and frustrating to both new and experienced users. The platform will support consistent production of high quality lentiviral particles at a very low cost for Manitoban researchers. It will also expand the users’ base of the existing Max Rady College of Medicine-funded biomedical functionality resource (that has housed four lentiviral vector shRNA libraries and two human ORFeome collections) on campus. The platform will facilitate discovery and validation of gene/protein functions in basic and translational research in Manitoba.
Core Platform for Histology, Histomorphology, and Ultrastructural Imaging (SIM, EM)
Location: Department of Human Anatomy and Cell Science, Max Rady College of Medicine
The histomorphology and ultrastructural imaging service platform offers:
In addition, we provide a service for structural illumination microscopy (SIM) for high-resolution fluorescent imaging of subcellular structures. The histomorphology and ultrastructural imaging platform will also offer educational modules, which include annual graduate student training courses in morphological techniques, histology, or ultrastructural imaging.
Flow Cytometry Core Platform
Location: 4th floor Apotex Centre and 5th floor BMSB
Aaron Marshall, Keith Fowke
Flow cytometry is well established as a clinical diagnostic tool and it remains an essential research tool for multiplex analyses of cellular biomarkers and isolation of rare cell populations. Recent advances in fluorescence labeling and instrumentation have dramatically advanced the analytical and cell sorting capabilities of flow cytometry. The flow cytometry core platform will create an integrated structure for management and promotion of flow cytometry technology. The core will support a wide range of applications including immunophenotyping and biomarker analyses of human samples and animal models, and a wide variety of cell function studies such as cell division, cell death, DNA content, enzyme activity, membrane permeability, ion and redox states. Flow cytometric isolation of rare cell populations based on up to 14 biomarkers is also available. Other services will include consultation on experimental design and data analysis. To educate the user base regarding available instrument capabilities and encourage uptake of the most powerful cell labeling technologies, the core will regularly organize educational workshops.
Core Platform in Mass Spectroscopy Analysis of Proteins and Metabolites
Location: Manitoba Centre for Proteomics and Systems Biology, 799 John Buhler Research
Mass spectrometry plays an essential role in protein discovery, quantitation, and monitoring of protein modifications, interactions and activities. This type of information is critical for the identification of biomarkers of disease activity or response to therapy and in the understanding of disease process. There are four instruments, two QqTOF for discovery-based projects and two triple quad instruments for quantitative analysis and analysis of posttranslational modifications. The latter instruments can also be used for small molecule quantitation. The Mass Spectrometry core platform will provide advice in experimental design, perform mass spectrometry and data interpretation. Workshops for students and researchers in the instrumentation and methodologies will be ongoing.
Central Animal Core Imaging and Transgenic Facilities (CACITF)
Location: BMSB 68-77 (SAMICF): JBRC (MTF)
SAMICF program Coordinator: Dr Mike Jackson
MTF Scientific Director: Dr Barbara Triggs-Raine
The Central Animal Core Imaging and Transgenic Facilities (CACITF) was recently established by combining two existing Central Animal Care Services facilities, the Mouse Transgenic Facility (MTF) and the Small Animal and Materials Imaging Core Facility (SAMICF). Integration of these two facilities provides researchers with a single point of access to facilities for developing new genetic models of disease states and applying advanced imaging techniques to these (and other) disease states.
Central Animal Care Services (CACS)
Location: BMSB , CHRIM, Chown & CancerCare Manitoba
Director: Robert Madziak
Central Animal Care Services (CACS) provides for the care, health and well-being of animals used for research and education at the Bannatyne Campus of the University of Manitoba. CACS provides for the animal related needs of University researchers and educators through dissemination of knowledge and resources, and ensures observance of all legal and ethical standards pertaining to the use of animals for research and education. Central Animal Care Services provides principal investigators with the support required to create the highest quality research environment while providing superior animal care.