PLNT7162

Plant Genomics

 

Course term: Winter, 2009

3 hrs lectures per week.

Instructor: Dr. Genyi Li

Office: 307 Agriculture building

Telephone: 474-7192

Email: g_li@umanitoba.ca

Office hours: by appointment

Grade methods

1 quiz in the middle of the term 20%

Presentation 25%

Group Discussion 25%

Final exam (Take home) 30%

The final examination will include questions and problems from lectures and assigned publications.

Note : Academic dishonesty is a serious offense. Please refer to the General Academic regulations and Policy section in the General Calendar for information on ‘plagiarism and cheating and examination impersonation’.

 

Plant Genomics Course #PLNT 7162

Course outline

 Introduction

Genome science involves mapping, sequencing and analyzing genomes. Structural and functional genomics are the cores of genome science. Proteomics and metabolomics lead to the further understanding of gene functions. Bioinformatics offers tools to mine the fast-accumulated data in genome science.

Structural Genomics

  • Molecular markers and genetic mapping.
  • Genome sequencing
  • High throughput sequencing

Gene expression analysis

  • Microarray
  • SAGE and MPSS
  • eQTL

Reverse genetics

1. Mutation and detection.

2. TILING (Targeting induced local lesions in genome).

3. RNAi (RNA interference).

4. VIGS (Virus induced gene silencing).

Gene cloning and functional analysis

  • Forward genetics
  • map-based gene cloning
  • QTL and cloning
  • Gene tagging with fluorescent proteins

Proteomics

  • Y2H and gene interaction
  • 2-D gel and protein purification
  • MS and protein identification
  • Protein modification, detection and functional analysis